Correlation Analysis Between Quantitative Monitoring Imaging Of DOX@ZrO₂ NP By Energy Spectrum CT And Real-time Drug Release

Objective:In this study,we design single-shell ZrO₂ NP?zirconium dioxide nano-particle?as a chemotherapeutic drug carrier,the construction of a new type of chemotherapy drug delivery system that allows real-time monitoring of particle absorption and release by gem energy spectrum CT imaging.We generated a standard working curve through quantitative analysis of the imaging to generate and,in vitro cell experiments concentration,analysis of imaging results reflect the feasibility of drug release,in order to evaluate the drug delivery and release in vivo and in vivo to provide imaging basis.Materials and methods:ZrO₂ NP was loaded with doxorubicin,a chemotherapeutic drug commonly used in clinical practice.The encapsulation efficiency and loading rate of chemotherapeutic drugs were calculated using an overdose method.In vitro real-time monitoring of doxorubicin release concentration,draw the drug release curve.Configure different concentrations of ZrO₂ NP solution,the use of gem CT scan spectrum sequence,the use of workstation processing analysis of the relative content of the base material to obtain different concentrations of ROI data corresponding to draw the working curve,draw a certain concentration range ZrO₂ NP.The relationship between the concentration of zirconium particles and the content of the base material measured in the ROI.HepG2 hepatocarcinoma cells were used as target cells and incubated for 24 hours with DOX@ZrO₂ at a concentration range of 0-800 ug/mL.The cytotoxicity of drug-loaded nanosized zirconium dioxide particles was evaluated using WST-8 cell counting kit?CCK-8?.HepG2 cells were incubated with DOX@ZrO2 solution at a concentration of 200?g/mL for different times,and intracellular adriamycin was determined by atomic absorption spectroscopy?AAS?.The cell suspension was analyzed by energy spectrum CT,the relationship between the imaging results and the nanoparticle concentration was analyzed,and the correlation between the imaging results and the intracellular drug concentration was analyzed.Results:The entrapment efficiency and loading rate of cryogenic centrifugal freeze-dried method were calculated by the method of overdose loading.The use of spectral CT-based material pair analysis can be used to monitor the concentration gradient of nanometer zirconium dioxide imaging curve and provide a reliable means of image monitoring for the transport and release of drug delivery system in vivo.According to the inhibitory effect of Zr O₂ NP on hepatoma cells,the slow release of appropriate concentration of drug particles in hepatocellular carcinoma cells was correlated with the imaging results of energy spectrum CT.Conclusion:The analysis results based on spectral CT scan images can reflect the actual drug concentration of the drug-loaded nanosized zirconium dioxide system in cells and provide a reliable means of image monitoring for drug transport and release in vivo.