The Expression And Clinical Significance Of REG4 And Survivin Protein In Ovarian Cancer Tissue

Objective:To study the relationship between regenerating islet-derived gene(regenerating islet-derived gene,REG)family's new member REG4 and apoptosis inhibiting factor(inhibitor of apoptosis proteins,IAP)protein family's new member survivin in epithelial benign ovarian tumor tissue and primary ovarian cancer tissue,and their correlationship with clinical pathological characteristics.To conclude their role playing in the occurrence and development of ovarian cancer.Methods: We select surgically resected ovarian tissues of 52 cases ranging from 2007 to2017 in gynaecology department of the fourth hospital affiliated to China medical university.52 cases include 13 cases of ovarian benign tumors and 39 cases of ovarian malignant tumors derived from epithelium.We collect their information of clinical pathological features.Immunohistochemistry(Elivision method)was applied on detecting REG4 and survivin protein expression level in 52 cases tissues.We use IBM SPSS Stastics software to analyze data with Spearman test,X2 test or Fisher test.We analyze the correlation between REG4 and survivin and how they relate to nature,type,differentiation degree,FIGO stage.Results: The REG4 protein expression rate of ovarian cancer tissues is 92.3%,which is significantly higher than that in the epithelium benign tumor tissue,which is 38.5%,P<0.05.The REG4 protein expression rate is 90.5% in clinical stage ?-? ovarian cancer tissues,which is higher than that in clinical stage ?-? ovarian cancer tissues,which is 61.6%,P<0.05.The protein expression rate of REG4 in the low differentiation ovarian cancer tissue is 90.5%,which is significantly higher than that in the middle and high differentiation ovarian cancer tissue(61.1%),P<0.05.The high protein expression rate of REG4 in serous ovarian cancer,mucinous ovarian cancer,clear cell ovarian cancer and endometrial ovarian tissue is 70.4%,85.7%,100.0% and 100.0% relatively,P > 0.05.In the 14 ovarian cancer tissues with routine lymph node dissection,the high expression rate of REG4 in lymph node metastasis and non-metastasis is 100.0% and54.5%,P>0.05.The protein expression positive rate 84.6% of survivin in ovarian cancer tissues is significantly higher than that in the epithelium benign tumor tissue,which is38.5%,P<0.05.The protein expression positive rate 90.5% of suvivin in clinical stage ?-? ovarian cancer tissues is significantly higher than that in the clinical stage ?-?ovarian cancer tissues,which is 55.6%,P<0.05.The protein expression rate of suvivin in the low differentiation ovarian cancer tissue is 95.2%,which is significantly higher than that in the middle and high differentiation ovarian cancer tissue(50.0%),P<0.05.The high protein expression rate of suvivin in serous ovarian cancer,mucinous ovarian cancer,clear cell ovarian cancer and endometrial ovarian tissue is 81.7%,42.9%,66.7% and100.0% respectively,P > 0.05.Among the 14 patients with lymph node dissection,survivin had a high protein expression rate of 100.0% and 72.7% respectively in the ovarian cancer with lymph node metastasis and non-metastasis,P>0.05.REG4 protein expression is proved to be positively correlated with survivin protein expression(r=0.375,P=0.019).Conclusion:1.The REG4 and survivin protein in ovarian cancer is highly expressed than in benign ovarian tumors.2.REG4 and survivin protein expression is associated with ovarian cancer staging and differentiation.The higher stage and the lower level of differentiation leads to the higher level of protein expression in ovarian cancer tissue.REG4 and survivin promotes the happening and development of ovarian cancer.3.REG4 and survivin are positively correlated with protein expression in ovarian cancer tissues,suggesting that they may jointly participate in the development of ovarian cancer.