The Protective Effect And Mechanism Of Ginsenoside Rg1 On C2C12 Cell Injury

Skeletal muscle atrophy was originally used to describe the term that muscle tissue lacked,it is now used to describe low muscle mass and low muscle strength and function.The quality of skeletal muscle is determined by the balance of protein synthesis and degradation,excessive protein degradation leads to muscle atrophy and increases morbidity and mortality.However,in the process of somatic movement or some pathological conditions,skeletal muscle is susceptible to injury and metabolic obstruction,affecting the normal life and action of the patients.Therefore,it is very important to treat and repair damaged skeletal muscle,maintain normal metabolism of skeletal muscle and improve the function of skeletal muscle.Ginsenoside Rg1,a panaxitriol,is one of the main active components in ginseng,it is widely used in the prevention of various diseases in oriental medicine.In recent years,ginsenoside Rg1is often used to prevent related diseases,Such as fighting inflammation,relieving fatigue,anti-aging and the protection of nervous system.Although several studies have shown that ginsenoside Rg1 has many important effects on human health,the role of it in skeletal muscle injury and its molecular mechanism are unknown.In this study,we had explored the protection and related mechanism of ginsenoside Rg1 for repair of skeletal muscle injury.[Experimental Objective]Explore the protective effect and mechanism of ginsenoside Rg1 on C2C12 cell injury.[Experimental Method]Using skeletal muscle cells of mice,which were cultivated with no serum?starvation treatment?,as the in vitro model of muscle cell injury.Skeletal muscle cells were incubated with different concentrations of ginsenoside Rg1.The damage repair effect of Rg1(10^-3,10^-2,10^-1,10¹mM)on C2C12cells was detected by using photomicrograph,cell counting and MTT;C2C12 cell protein expression was detected by BCA;Western blot was used to detect the expression of ribosomal protein S6 kinase P70S6K and the initiation factor 4E binding protein1?4E-BP1?.LY294002 and rapamycin,inhibitors of the P13K and the mTOR,respectively,were used to treat cells,and then detecting the phosphorylation levels of P70S6K,AKT,mTOR and 4E-BP1.[Experimental Results]After C2C12 of skeletal muscle cells of mice were treated with different concentrations of Rg1,the number of C2C12 cells and cell vitality were significantly increased compared with the control groups.Rg1 could improve the mass of protein expression when C2C12 cells injured,inhibit the phosphorylation of4E-BP1,activate phosphorylation of the ribosomal protein S6 kinase P70S6K,and activated signaling pathway of the P13K-AKT-mTOR.The function of Rg1 in signaling pathway of the P13K-AKT-mTOR could be inhibited by LY294002 and rapamycin.[Experimental Conclusion]Ginsenoside Rg1 can promote the repair after damage of the skeletal muscle myoblasts C2C12 in mouse,indicating Rg1 involves in protein regulation of skeletal muscle cells,and the control relies on the signal path PI3K-AKT-mTOR,Rg1 ginsenosides in repair after injury of skeletal muscle,muscle atrophy and disease treatment and improve the clinical research and application of skeletal muscle function and so on provides the theoretical basis and new ideas.