Ginsenoside Rg1 Inhibition Of Protein Degradation And The Mechanism Of Muscle Cell C2C12

Skeletal muscle is the connective tissue,to maintain the body posture and body movement are extremely important role.Skeletal muscle also is a kind of highly plastic tissue,can produce significant changes to various external stimuli.The skeletal muscle fibers cannot undergo mitosis,the plasticity of regulating the protein level regulation belongs to the division after such as degradation of skeletal muscle atrophy is greater than normal protein metabolic conditions caused by protein synthesis.Therefore,regulation of protein synthesis and degradation of skeletal muscle balance can effectively prevent muscle atrophy.Ginseng is Panax ginseng root plant,is the precious medicinal herbs commonly used in traditional medicine.Ginseng with pharmacological action and the biological activity in many aspects,contains many kinds of chemical composition types,is one of the most important active components of ginsenosides.Ginsenoside Rg1 is three alcohol type B type ginseng saponin,for God The system,immune system,cardiovascular system have protective effect,also can inhibit cell apoptosis,anti-aging and functional recovery of sports fatigue.Recently discovered,Rg1 on treatment of muscle atrophy also certain effect.Therefore,this experiment used starvation treatment method of C2C12 cells cultured in serum free medium,making muscle atrophy models and detection Rg1 anti muscle atrophy and cell protective effect and the role of molecular mechanism.The purpose of the experiment was to study the effect and mechanism of Rg1 on the anti muscle atrophy at the molecular level.Used a mouse subculture skeletal myoblast cell line C2C12 cells,by the MTT assay and morphological observation method,and tested different concentrations Rg1 effects on cell viability.By flow cytometry and confocal microscopy was used to detect Rg1 on C2C12 cell apoptosis.By Western blot Western blot method to detect Rg1 on C2C12 cells,ubiquitin ligase enzymes that affect the expression of E3.Changes inhibitor LY294002 treated cells were detected Rg1 on C2C12 cells AKT,FoxO1/3a,mTOR phosphorylation levels and other substances while using PI3 K,and to determine the above changes PI3 K relevance.C2C12 cells were incubated Rg1 can improve cell activity.At the same time can inhibit the expression of cell E3 ubiquitin ligase,activation of AKT,FoxO1/3a,mTOR phosphorylation,more action Rg1 have been inhibited by an inhibitor LY294002.Based on the above experimental results show that Rg1 can resist free serum culture induced cell activity decreased and apoptosis;can inhibit the expression of ubiquitin ligase E3,and this effect is through a PI3 K dependent AKT/FoxO1/3a signaling pathway.This study for clinical treatment of muscle atrophy of shrinkage,prevention and prognosis assessment provide new ideas and methods.