Study On Single Nucleotide Polymorphisms Of P73 Gene In Ovarian Tumors

Objective:To explore the relationship between Single nucleotide polymorphism(SNP)and ovarian cancer,and to provide an effective biological indicator for the early in the development of ovarian cancer and disease progression.Methods:From May 2016 to May 2017,a total of 110 patients who underwent surgical treatment in the gynecological department of tumor hospital of yunnan province were collected.Ovarian tumor,96 cases(65 cases,malignant border sex:13 cases of benign 17 cases)and non ovarian tumors(for uterine fibroids,and ovarian abscess,ovarian endometriosis)appendix resection in patients with 14 cases.After the removal of the ovarian tissue,the tubal and venous blood,the PCR amplification of DNA was extracted,and the genotype of P73 gene rs6695978 was detected by gene sequencing.And study the relationship between different genotypes and ovarian cancer risk and clinical pathology.To compare the genotype distribution of different types of tumors.Results:1.Gene sequencing results showed that 110 patients were detected,with A total of 35 cases of patients with A single base from G base displacement as A base,including the control cases in 2 cases,3 cases of benign tumor group cases,border tumor group 4 cases,26 cases of ovarian cancer group.The alleles of the two patients were all from G base to A base,all of which were cases of ovarian cancer.2.The results of the Hardy-Weinberg equilibrium test show,the distribution ofP73 gene rs6695978 in the benign tumor group,the borderline tumor group,the ovarian cancer group and the control group was consistent with the Hardy-Weinberg equilibrium(P>0.05),The samples of this experiment are representative of the group.3.P73 gene test results showed that the allele frequency and genotype frequency of rs6695978 were not statistically significant between the benign tumor group,the borderline tumor group,the ovarian cancer group and the control group.4.According to pathologic types,stages,lymph node status,the group ovarian cancer stage points ?-? with ?-? group,the serous adenocarcinoma group and other group,the mucous adenocarcinoma group and other groups,the positive and negative lymph node lymph node and hierarchical analysis.Results showed that rs6695978 P73 gene loci(G>A)G allele and A allele frequency in ? stage-? with?-? comparative differences between groups was statistically significant(P =0.039),and compare differences between lymph node positive lymph node negative group was statistically significant(P = 0.046).The alleles and genotypes of the P73 gene rs6695978 were not statistically significant(P>0.05)in the first diagnosis age group with the age of 50 and older than 50 and the serous adenocarcinoma group and the other groups.5.In the three tissues of the same patient,there were differences in the mutations of P73 gene rs6695978 site,the highest mutation rate of ovarian tissue,and the mutation rate of the tissue mutation rate of-the oviduct.Conclusions:1.The polymorphism of SNP locus rs6695978(G>A)in P73 gene has no apparent correlation with the risk of ovarian tumor's occurrence and development.2.The polymorphism of SNP locus rs6695978(G>A)in P73 gene has no apparent correlation with the risk of ovarian attack,while it relates to ovarian metastasis.It is speculated that patients with A gene may be at greater risk for pelvic and lymph node metastasis than those carrying the G gene.In addition,prognosis is worse.3.The polymorphism of SNP locus rs6695978(G>A)in P73 gene has no apparent correlation with initial diagnosis and pathologic type of the patients with ovarian cancer,It's necessary to mention that the polymorphism of SNP locus rs6695978(G>A)in P73 gene exists in ovarian cancer patients of different ages and various pathological types.4.Among all tissues with the polymorphism of SNP locus rs6695978(G>A)in P73 gene,ovarian tissue is apparently more than oviduct tissue.There are only 6 cases that G base is displaced by A base in both ovaries and fallopian tubes.It's necessary to mention that the genetic polymorphism of the ovaries and fallopian tubes was not consistent in this experiment.5.The polymorphism of SNP locus rs6695978(G>A)in P73 gene belongs to acquired gene mutation which is also called systematic mutation.