| [Objective] Micro RNA-422a(mi RNA-422a)is under-expressed in several types of tumors,which can inhibit the proliferation and invasion of tumor cells.Our previous study was to investigate the role of microrna-422 a in the proliferation and apoptosis of osteosarcoma cells(MG63)induced by paclitaxel.[Methods] 1.The mi R-422 a mimics was transfected into MG63.The experiment was divided into 4 groups:blank control group,mi RNA-422 a group,paclitaxel group and mi RNA-422 a + paclitaxel group.After transfection,transfection efficiency was observed by GFP.2.Mi RNA-422 a expression level was detected by RT-PCR.3.Cell proliferation was detected by MTT proliferation assay.4.Cell apoptosis were detected by flow cytometry.[Results] 1.The mi RAN-422 a group,mi RNA-422 a + paclitaxel group were observed to be green fluorescence,and the transfection efficiency was more than 70%.No green fluorescence was observed in the blank control group and paclitaxel group.2.Compared with blank control group,the mi RNA-422 a in mi RAN-422 a group,mi RNA-422 a + paclitaxel group was significantly increased(P<0.05).Compared with paclitaxel group,the mi RAN-422 a in mi RNA-422 a mimics + paclitaxel group was significantly increased(P<0.05).3.Compared with the blank control group,cell proliferation rate in mi RNA-422 a mimics,paclitaxel,mi RNA-422 a mimics+paclitaxel group were significantly decreased(P<0.05),compared with paclitaxel group,cell proliferation rate in mi RNA-422a+paclitaxel group was significantly decreased(P < 0.05).4.Compared with the blank control group,cell apoptosis rate in mi RNA-422 a mimics,paclitaxel,mi RNA-422 a mimics+paclitaxel group were significantly increased(P < 0.05),compared with paclitaxel group,cell apoptosis rate in mi RNA-422a+ paclitaxel group was significantly increased(P < 0.05).[Conclusion] Microrna-422 a can effectively inhibit the proliferation of osteosarcoma cells and synergize with paclitaxel to promote apoptosis of osteosarcoma cells. |
