The Clinical Significance Of Combining Analysis Of Colorectal Cytology And Colposcopic Histopathology As Well As Expression PD-L1 On Cervical Lesions Tissue

Part IObjective: The study was to explore the detection significance of liquid-based cytology test combined with pathological under colposcopy examination for cervical diseases.Methods: In accordance with the established inclusion criteria and exclusion criteria,from November 2014 to July 2017,386 cases of our hospital having TCT,colposcopy histopathological examination of cervical disease patients from November to 2017 In July,104 cases of TCT were examined by colposcopic histopathological examination to screen the results of patients with cervix disease.Results:TCT examination abnormal tissue pathology colposcope positive rate was42.3%,significantly higher than the histopathological examination and colposcopy simultaneously TCT 17.1%,a significant difference is statistically significant(p <0.05).Conclusion: Cervical TCT abnormalities were performed colposcopy histopathological examination than at the same time cervical TCT and colposcopy histopathological examination has a higher diagnostic compliance rate;while the cervical spine TCT and colposcopy histopathological examination,and Did not improve the detection rate of cervical intraepithelial neoplasia,but increased the patient’s cervical trauma and economic burden.Therefore,cervix TCT cytology abnormalities and then colposcopy histopathology is the current screening of cervical cancer and precancerous lesions of the main means.Part IIObjective: Immunohistochemical assay(streptavidin-perosidase)was applied to study PD-L1 expression in chronic inflammation and cervical intraepithelial neoplasia(CIN).The correlation between PD-L1 and clinicopathological features was studied to find out their association with the development of cervical cancer,which provided experimental basis for diagnosing and treating cervical precancerous lesions.Therefore,cervical cancer incidence and mortality rates could be reduced by incorporating comprehensive susceptibility group differences and optimal combination screening program.Methods: 111 patients(June 2001 to April 2017)were enrolled and paraffin-embedded specimens were collected by surgical resection or biopsy.PD-L1 expression was detected in 48 cervical chronic inflammation and 63 cervical intraepithelial neoplasia patients.The relationship between PD-L1 and clinicopathological features was analyzed together with individual patient data to discover its value in diagnosis and treatment of cervical precancerous lesions.Results: PD-L1 was detected positive among 66.67% of chronic inflammation patients.Its positive rate in the CINⅠ group was 96.43% and 97.14% in the CINⅡ-Ⅲgroup.Leiomyosarcoma had a negative result.96.83%(61/63)PD-L1 expression in CIN group was significantly higher than that in chronic inflammation group(66.67%),which showed statistical significance(p<0.05)Conclusion: Cervical cytology with colposcopic histopathological examination is one of the major methods for screening and diagnosing cervical cancer and precancerous lesions,which can improve the diagnosis of cervical intraepithelial neoplasia and reduce misdiagnosis rate.PD-L1 was seldom expressed in normal cervical tissues,but over-expressed in high-grade lesions.It showed more expression level with the increased lesion degree indicating PD-L1 expression was related to the severity of cervical lesions.In this study,PD-L1 expression analysis by immunohistochemical SP could provided a new biomarker for the examination of cervical lesions.