The Research On The Relationship Between The Serum Mast Cell Carboxypetidase Levels And Bronchial Asthma

Objective To investigate the function of the mast cell carboxypetidase in the bronchial asthma through detecting the serum The mast cell carboxypetidase levels of asthma patients,and further study the relationship between The mast cell carboxypetidase concentration and pulmonary function ?FENO or IgE.Methods there are 70 person with asthma of Ji Lin University First Hospital were recruited into this study as the case group.These person were treated with 2mg budesonide inhalation?0.4mg salbutamol and 2.5ml Ipratropium Bromide aerosol inhalation(Once every eight hours)and 0.3g doxofylline twice one day,take orally 10 mg montelukast once a day before sleep,regiments for seven days.They need to inhale budesonide/formoterol 160/ 4.5ug twice one day and tiotropium bromide 4.5ug once a day at eight o'clock p.m keeping 3 months after leaving the hospital and to attain clinical control phase.Divideding the experimental group into IgE increased group(n=43)and IgE normal group(n=27)according to the IgE level.healthy controls including 21 volunteer recruiting from the Ji Lin University First Hospital,The mast cell carboxypetidase were measured using ELISA.The mast cell carboxypetidase concentration and the lung function also measured pretherapy and post-treatment,and comparing the mast cell carboxypetidase levels between the asthma patients at acute attack phase or clinical control phase and healthy controls.The correlation between the mast cell carboxypetidase levels and pulmonary function was further analyzed.Rrsults 1?In clinical cases of bronchial asthma remission the MC-CP,FENO level significantly lower than the acute phase(P < 0.05),while higher than healthy controls (P < 0.05);And FEV1%pred levels in clinical controls obviously higher than that of the acute phase,there was no evident difference with the normal group(P = 0.281)2?The level of plasma mast cell carboxypetidase of the person in IgE increased group was higher than that in IgE normal group(P<0.05)either the acute episode of bronchial asthma or the clinical remission period.3?There was a significant negative correlation between serum MC-CP and FEV 1(%)in the patients with acute episode of bronchial asthma(r=-0.426,P < 0.001),while the clinical remission period was not correlated(r=-0.221,P=0.066).There was no correlation between serum MC-CP and FENO in patients with both the acute episode of bronchial asthma and clinical controls(r=0.054,P=0.659;r=.137,P=0.257).4?The serum MC-CP was significantly negatively correlated with FEV1%pred in both IgE increased group and IgE normal group of patients with acute episode of bronchial asthma(r=-0.429,P=0.004,r=-0.004,613,P=0.001).In IgE normal group with acute episode of bronchial asthma,the serum MC-CP and FENO were significantly positively correlated(r=0.412,P=0.033),while there was no correlation in IgE increased group(r=-0.265,P=0.086).Conclusions 1?The plasma mast cell carboxypetidase concentration in Bronchial asthma patients at acute attack phase was obvious increased than that in healthy controls,and the serum mast cell carboxypetidase concentration of the patients in increased IgE group was higher compared with the normal IgE group,concluding that The mast cell carboxypetidase could participates in the airway inflammatory response of the asthma acute phase;2?The serum level of mast cells carboxypeptidase in clinical remission of bronchial asthma still significantly higher than that of healthy controls,existing a certain level of inflammation in the body of Bronchial asthma patients at clinical remission;3?There was a obvious negative correlation between the serum mast cell carboxypetidase level and FEV1%pred in asthma patients at acute attack phase.