Association Between Genetic Polymorphisms Of Ghrelin And Metabolic Syndrome

The metabolic syndrome(Met S)is a complex syndrome which is composed of protein,fat and carbohydrate metabolic abnormalities.It includes abnormal lipid metabolism,elevated blood pressure,central obesity and raised glucose.The occurrence of the metabolic syndrome is closely related to the lifestyle,dietary habits and genetic factors.It may increases the risk of developing cardiovascular disease,cancer,type 2 diabetes,sleep apnea syndrome,polycystic ovary syndrome,non-alcoholic fatty liver disease and dementia.ObjectiveThis study assessed Ghrelin SNPs for association with a risk in developing metabolic syndrome in Han Chinese population.MethodsA total of 3850 Han Chinese subjects were included in this case-control study,including 1813 cases(Met S)and 2037 healthy controls and their age and gender were matched.Extraction in peripheral blood of all subjects,three SNPs(rs10490816,rs696217 and rs20802)were genotyped using the technology of PCR and MALDI-TOF-MS.The association between Ghrelin SNPs and Met S was analyzed statistically by SPSS 22.0 and SNPStats.Results1.A total of 3850 Han Chinese subjects were included in this study,among them,there were 1813 cases(903 males,50.2%,910 females,49.8%,)and 2037 controls(1024 males,50.3%,1013 females,49.7%),the average age of the case group was 49.55 ±9.73 and the average age of the control group was 49.60±9.41.There was no significant difference in the gender(?2=0.052,P=0.796)and age(t=0.065,P=0.950)between the case and control groups.2.We found that rs26802 and rs696217 were in accordance with Hardy-Weinberg Equilibrium(P>0.05),but rs10490816 genotype frequency did not conform to the HWD test(P<0.05).There was no statistical difference in allele and genotype frequency of rs26802 between the case and control(?2=0.209,P=0.669;?2=1.093,P=0.579)and the best model for this SNP was recessive model;There was no statistical difference in allele and genotype frequency of rs696217 between the case and control(?2=0.220,P=0.649;?2=1.338,P=0.512)and the best model for this SNP was recessive model;There was statistical difference in allele frequency of rs10490816 between the case and control(?2=29.702,P<0.001),but there was no statistical difference in genotype frequency of rs10490816 between the case and control(?2=1.005,P=0.605)and the best model for this SNP was recessive model;3.There was also no haplotype that could be associated with the risk of developing Met S(P>0.05).4.SNP rs10490816 of Ghrelin was associated with triglyceride(CC>CG>GG,F=2.047,P=0.045)and total cholesterol(GG>CG>CC,F=3.328,P=0.006),while SNP rs26802 was associated with BMI(GG>GT>TT,F=3.991,P=0.010),and waist circumferences(GG>TT>GT,F=6.180,P=0.022),DBP(TT>GT>GG,F=62.680,P <0.001)and fasting glucose(TT>GG>GT,F=35.311,P <0.001).SNP rs696217 was associated with SBP(TT>GT>GG,F=3.184,P=0.042),fasting glucose(TT>GT>GG,F=39.342,P <0.001)and hip circumferences(GG>TT>GT,F=2.917,P =0.012).Conclusion1.Based on our data,we found no association between rs696217?rs26802 and rs10490816 polymorphisms and risk of metabolic syndrome,and there was no association between any haplotypes and Met S.2.We found that SNP rs10490816 of Ghrelin was associated with triglyceride and total cholesterol,while SNP rs26802 was associated with BMI,waist circumferences and fasting glucose,SNP rs696217 was associated with SBP,fasting glucose and hip circumferences.3.The Ghrelin may not be a susceptible gene for metabolic syndrome,but the gene may be associated with the metabolic syndrome related factors.