Exploration Of The Relationship Between Urinary Calculus And Intestinal Flora

Part I: To clarify the relationship between urinary calculus and intestinal floraPurpose: To clarify the relationship between urolithiasis and intestinal flora.Methods: This study consists of three parts.1.Collect fecal samples from patients with urinary stones from March 2017 to December 2017 in the experimental group.Fecal samples from patients without urinary tract stones in orthopedics department as control groups.The samples were sequenced for 16 S to analyze the species of the two strains difference.After cryopreservation,the specimens were removed and dissolved in water.The supernatant was used as an experimental backup.2.Make urinary stones model with 1% ethylene glycol water.At the same time as the modeling,mice were given fecal gavage for three weeks.Grouping and feeding mice with different components of water(1 stone water + feces of people with urolithiasis;2 stone water + mouse feces;3 stone water + normal people's feces;4 Stone water + normal saline)four weeks.3.Dissect and observe the number of crystals in the renal tubules of the mouse kidneys,find the ratio of the total number of renal tubules observed in the visual field,classify and summarize,calculate the average value of each group,and calculate the difference.Results:1.The intestinal flora of patients with urinary stones differs from the general population,regardless of the abundance of the flora or the flora species.Compared with normal control group,the bacterial population abundance of stone patients was significantly reduced.The proportion of Bacteroides and actinomycetes increased significantly in patients with stones.2.In the mouse model,the incidence of urolithiasis was 0.18% in the experimental group(stone water + feces of people with urolithiasis);it was significantly higher than 0.09% in the stone++ mouse feces group and 0.08% in the stone+normal people's feces group.,Stone water + normal saline group 0.05%(p<0.05).Conclusion:The intestinal microflora of patients with urinary calculi are different from those of the general population.This difference can increase the speed of the formation of crystals in the renal tubules of patients with urinary calculi,thus accelerating the formation of their urolithiasis.So that they grow stones faster than normal people.However,the individual size of the mice fed with human fecal supernatant and the control group also showed a significant difference.The specific reasons and related mechanisms have yet to be further elucidated.Part II: Analyze and select the strains that may affect the intestinal flora produced by stones.Purpose: To analyze and select the strains that may be involved in slowing stone formation and try to explain its possible mechanismMethods: 1.Collect fecal samples from patients with urinary stones and feces of normal people and sequence them for 16 S to analyze the species of the two strains difference.2.Adult mice of healthy adult age and similar body weight were divided into two groups.Rat feces samples were collected brfore perfusion of human feces.After labeling,they were stored at low temperature.After the two groups were divided to feed fecal samples from patients with perfusion stones and fecal samples from normal persons.The solution was taken for three weeks and samples of both their feces were taken for preservation.3.The mice feces samples were collected from feces before and after 3 weeks of perfusion.The fecal flora of the mice was analyzed.The bacterial populations in each group were compared to select the strains that may affect the formation of urolithiasis.Results:In the experiment,there was no significant change in bacterial abundance before and after 3 weeks of perfusion.Dorea formicigenerans were significantly reduced in the feces of stone cases compared with normal people.After the gavage of patients with gurgling stones in the mouse,this strain also showed a significant reduction.It is speculated that the two strains are closely related to the formation of stones.Dorea formicigenerans is a gram-positive obligate anaerobe,and acetic acid,formic acid and acetic acid lactic acid and ethanol are the main products of its metabolic glucose production.Pyruvate can be converted to acetate,formate and ethanol,lactic acid and gluconate can not be used.Threonine does not convert to propionic acid.Nitrate is not degradable.Does not metabolize to produce quinones.Conclusion: Abnormal intestinal flora is closely related to the formation of urinary stones.Dorea formicigenerans may be involved in the formation of urinary calculi,but the specific influencing factors and the mechanisms involved in metabolism remain to be further studied and proved by experimental evidence.