Protective Effect Of Edaravone On Lung Injury Induced By Blast Injury In Mice

Objective:In recent years,the number of international and domestic explosions has been increasing,so that the damage caused by the explosion is no longer confined to the battle field but to the people's life.Most of the explosions in our country took place in the explosions of industrial production and flammable and explosive materials,and the incidence of explosions increased year by year.Detonation injury occurs mainly at the interface between air and tissue,that is,gas-bearing organs are more likely to be affected.In addition to auditory organs,the lungs are considered as the most common organ in primary injury and the most common cause of death in patients with primary blast injury.A series of inflammatory reactions occur in the lung injury induced by shock wave,release a large number of inflammatory cytokines,accompanied by oxidative stress reaction,aggravating lung injury.Edaravone exerts lung protection due to its antioxidant and anti-inflammatory properties.The purpose of this study was to investigate the protective effect of edaravone on the expression of inflammatory and oxidative stress factors in lung injury mice induced by blast injury,to provide a reference for clinical prevention and treatment of lung injury caused by explosive injury.Methods:1.Establishment of blast lung injury model: a detonation injury device is made according to the literature data,the lower part is the air compression device,the lower air compression device compresses the air,and blast wave is generated when certain pressure is reached.After weighing the mice,the mice were anesthetized,and the anesthetized mice were placed on the top network device of the device,exposing the thorax,and then producing shock wave to the mice after electrified,and making a blast lung injury model.2.The animal grouping: Forty male Kun ming mice(6-8 weeks)were randomly divided into four groups(n=10).They were normal control group(group A),injury group(group B),edaravone prevented group(group C)and edaravone treated group(group D).Except for group A,the other mice in each group were given a moderate lung injury model.Ten minutes before blast injury,Group C was given intraperitoneal injection of edaravone 15 mg/kg.Thirty minutes after blast injury,Group D was given intraperitoneal injection of edaravone 15 mg/kg,Group B was given an equal volume of 0.9% sodium chloride 30 min after injury,and group A was untreated.3.Drawn and testing: Blood samples were collected from venous blood of each group at 24 h after injury to detect the contents of tumor necrosis factor(TNF-?),interleukin-6(IL-6)and interleukin-10.Left lung tissue was collected for lung tissue homogenate and the activities of malonic dialdehyde(MDA),superoxide dismutase(SOD),myeloperoxidase(MPO)and NF-?B were determined.Take part of the left lung tissue pathological hematoxylin-eosin(HE)staining sections observed by light microscopy.Results: 1.The observation of pathological of lung tissue: Under the light microscope,the alveolar structure was complete in normal control group(group A),the alveolar wall was uniform,the alveolar cavity was little exudate and no erythroblasts exuded.The injury group(group B)showed diffuse alveolar hemorrhage,a large number of neutrophil infiltration in lung tissue,edema fluid filling or alveolar collapse,pulmonary interstitial edema significantly.In edaravone treatment group(group D),a small amount of hemorrhage of alveolar space was observed in the blisters,the neutrophil infiltration in the lung tissue was alleviated and the interstitial edema in the lung was relieved.The alveolar structure of edaravone prevented group(group C)is more clear,only has a small amount of neutrophil infiltration in lung tissue.2.The serum levels of TNF-?,IL-6 and IL-10: Compared with injury group(group B),the levels of TNF-? and IL-6 in group D were significantly decreased(P <0.05)and the level of IL-10 was significantly increased.And the above-mentioned changes were more obvious in edaravone prevented group(group C)(P <0.05).3.Lung tissue of NF-?B : Compared with injury group(group B),NF-?B was significantly decreased in edaravone treatment group(group D)(P <0.05),and the above-mentioned changes were more obvious in the edaravone prevention group(group C)(P <0.05).4.Lung tissue of MPO,MDA,SOD: Compared with injury group(group B),the MDA and MPO were significantly decreased in edaravone treatment group(group D)(P<0.05),SOD increased significantly(P<0.05),and the above-mentioned changes were more obvious in the edaravone prevention group(group C)(P <0.05).Conclusion: 1.The lung injury caused by blast injury resulted in obviously bleeding and edema of lung tissue,the destruction of the alveolar structure,the extremely uneven alveolar septum and a large number of inflammatory cell infiltration.The edaravone treatment group(group D)and prevention group(group C)could reduce the injury.2.Edaravone play a protective role against blast lung injury by anti-oxidant effect,and the preventive medication is better.3.Edaravone can control the release of inflammatory cytokines by inhibiting NF-?B and can reduce the degree of inflammation of lung injury caused by shock wave.Its protective mechanism is associated with down-regulation of inflammatory cytokines and up-regulation of the expression of anti-inflammatory cytokines.