Astragaloside? Regulates The Expression Of Inflammatory Factors And Oxidative Stress In PM2.5-induced Lung Injury Via P38MAPK/ERK/NF-?B Signaling Pathways

Objective: To explore the protective effect and mechanism of Astragaloside on lung tissue of PM2.5-induced lung injury rats,and to provide ideas for further study of astragaloside,and to provide new experimental basis for the prevention and treatment of pm2.5-induced lung injury.Methods: 35 SD rats(male,SPF grade)were randomly divided into normal saline control group,PM2.5 model group,high dose Astragaloside IV group,low dose Astragaloside IV group and SB203580 group.In order to observe the protective effect of Astragaloside on PM2.5 lung injury,preventive administration was used in this experiment.Intraperitoneal injection(i.p)was given 3 days before modeling,once a day for three consecutive days.The preventive dosage was 100 mg / kg.b.w,50 mg / kg.b.w,and 1mg / kg.b.w for Astragaloside IV high dose,and 1mg / kg.b.w for SB203580.The normal saline group and the model group were given the same volume of normal saline.30 minutes after the last prophylactic administration,the first model was made by intratracheal instillation(i.t)of PM2.5 suspension.The second PM2.5 intratracheal instillation was given on the 5th day after the first prophylactic administration(i.e.the 8th day of the whole experimental cycle).At the time node of 12 hours after the completion of PM2.5 infusion twice,rats were killed by pentobarbital sodium anesthesia combined with femoral artery bleeding and lung tissues were taken..HE staining was used to observe the pathological changes of lung tissue;W/D ratio and and total lung water content were used to evaluate the condition of pulmonary edema;Luminex high-throughput multi cytokine kit was used to measure the expression of IL-2,IL-4,IL-6,IL-10,ICAM-1,IFN-?;SOD,CAT,GSH-Px,MDA and MPO were used to evaluate the changes of oxidative antioxidant level;WB was used to measure the table of P-P38 MAPK,p38MAPK,p-ERK,p-NF-?B,p-I?B The expression and distribution of p-P38 MAPK,p-ERK and p-NF-?B were detected by immunohistochemistry.SPSS 22.0 was used for data statistics and analysis,and Graph Pad prism 8.0 was used for mapping.Results:(1)Astragaloside IV can inhibit PM2.5-induced lung tissue damage,inflammation and edema.(2)Astragaloside IV can reduce the increase of IL-2,ICAM-1 and IFN-? in lung induced by PM2.5,and up regulate the expression of IL-4,IL-6 and IL-10.(3)Astragaloside IV can up regulate the expression of SOD,GSH PX and CAT in the antioxidant enzyme system of PM2.5-induced lung injury,and reduce the expression level of MPO and MDA.(4)Astragaloside IV can inhibit the activation of p38 MAPK / ERK and NF-?B signaling pathway in PM2.5-induced lung injury,and alleviate inflammation and oxidative stress injury.Conclusion:(1)Astragaloside IV plays a protective role in PM2.5-induced lung injury;(2)Astragaloside IV protects PM2.5-induced lung injury through antiinflammatory and antioxidant effects;(3)Astragaloside IV may play a protective role by regulating p38 MAPK / ERK and NF-?B signaling pathway.