Study On The Anti- Oxidative Effect Of Edaravone For Lung Cancer A549 Cells Which Oxidative Damaged By UVC

Objective: To investigate the anti- oxidative effect of edaravone by establishing UVC-induced oxidative damage model of human lung cancer cells A549 and applying edaravone to treat the model.Methods: 1. The human lung cancer A549 cells were treated with different doses of ultraviolet UVC(UVC0:0J/m2,UVC1:10J/m2,UVC2:20J/m2,UVC3:40J/m2,UVC4:80 J/m2),Cell viability was evaluated by MTT assay.After that,the best condition of the manufacture of oxidative damage model in A549 was determined. 2. The A549 cells model was cultured by F12 medium with different concentrations of edaravone(Eda0 group:0μmol/l,Eda1 group:10μmol/l,Eda2 group:20μmol/l,Eda3 group:40 μmol/l,Eda4 group:80 μmol/l).The proliferation rate of cells in each group was evaluated by MTT assay.After that,the best concentration of edaravone was determined. 3. Through the above experiment,the best condition was determined.Then the extent of oxidative damage A549 cells was tested.Experimental cells were divided into three groups:blank control group,UVC group and UVC+Eda group.The level of MDA,the viability of SOD and GSH-PX was measured. 4. That the optimal concentration of edaravone impacts on DNA oxidative damage of A549 cells caused by different doses of UVC was tested by comet assay.Group A(UVC damage group)and group B(the optimal concentration of edaravone treatment group) was divided.Results: 1. As the ultraviolet radiation dose increased, the survival rates of cells isdecreased.The optimal condition of ultraviolet irradiation which is 40J/m2 was determined by MTT. 2. Different concentrations of edaravone could improve the appreciation rate of A549 cells and the appreciation rate of Eda3 group was the largest. After that, the best concentration of edaravone which is 40 μmol/l was determined. 3. Through the above experiment,the best condition was determined.Then the extent of oxidative damage A549 cells was tested.Experimental cells were divided into three groups:blank control group,UVC group and UVC+Eda group.The level of MDA and the viability of SOD and GSH-PX was measured. 4. That the optimal concentration of edaravone impacts on DNA oxidative damage of A549 cells caused by different doses of UVC was tested by comet assay.Group A(different doses of UVC damage group)and group B(the optimal concentration of edaravone treatment group) was divided.Conclusion: Edaravone could significantly reduce the oxidative damage effect induced by oxidation damage in lung cancer A549 cells.