| Objective : In this study,through the "ShuXueJieYuFang" treatment of depression model rats,compared with model group and acupuncture group in the hippocampus of rats weight changes,the content of Ras protein in Erk pathway changes,hippocampus nerve cell changes,from a macroscopic study on acupoint Jieyufang effect on sea horse weight of the rat model of depression,from the microscopic study solution depression prescription on hippocampal Erk pathway in neurons and Ras protein,and to explore the optimal solution mechanism of acupoint prescription treatment of depression depression,for the clinical promotion of "Jieyu Decoction" in treating depression and provide laboratory evidence for more reliable.Methods : First,the number of horizontal movement and the number of vertical movements were selected by the open box experiment in the 30-120 rats,and the others were removed,Then 30 SD rats were randomly divided into 3 groups,each group of 10 rats.Group A: blank control group;group B: model group;group C: Acupuncture group.Group A as control group,without any treatment;,B group and C group began to receive three weeks of different stress stimulation: swimming in cold water(4?,5min),(24h),fasting water deprivation(24h)and night(24h)and heating(45?,5min),bound(3h),tail clamp(3min).The above stress stimuli were randomly selected for 1 kinds,each of which was 3-4 times.In the acupuncture group,the C group was treated with the conventional acupuncture point after the model was made,and the Hua Tuo 0.25mm×25mm acupuncture needle was selected for acupuncture,and the stimulation was stimulated by using the SDZ-II electric stimulator.The use of electrical stimulation and not excessive stimulation in rats leads to the struggle,scream,in general with the point of skin around vibratiuncle is appropriate,the current should be kept below 2mA,for 20 min,1 times / day,acupuncture treatment of 21d;Group A was not given stress stimulation and acupuncture treatment,B group only took stress stimulation without acupuncture treatment.After the end of the experiment,through the observation of the morphology of the hippocampus and the measurement of the weight,the results of the laboratory data were compared;Enzyme linked immunosorbent assay(ELISA)was used to detect the changes of RAS protein in different groups,and the changes of neurons in hippocampus were observed under light microscope.Results:1.We can see that there are no significant differences in the body weight,the consumption of sugar and the carton experiment before the experiment(p>0.05).(1)21days after weight change: compared with A group,the weight of rats in B group decreased significantly(P<0.01)after the experiment for twenty-first days;Compared with B group,the weight of rats in C group increased significantly after twenty-first days of experiment(p<0.01).(2)21 days later,the experiment of sugar water: compared with A group,the consumption of sugar in the B group was significantly lower(P<0.01);compared with the B group,the degree of preference of sugar in C group was significantly higher(p<0.01).(3)after the experiment,the experiment of carton: compared with the A group,the level of and the vertical motion score of the rats in B group were significantly decreased,and the results were significantly different(P <0.01);Compared with B group,the rats in group C were significantly higher in both horizontal and vertical movement(P <0.01).2.The effect of acupuncture point "Jieyu prescription" on hippocampus of stress model rats is as follows:Compared with the blank group,the weight of the hippocampus in the model group was not statistically significant(P > 0.05),but the content of Ras protein in the Erk pathway was decreased and the number of nerve cells was decreased.The results showed that there was significant difference(P < 0.01);Compared with the model group,the expression of Ras protein in hippocampal neurons and the protein in the Erk pathway decreased in the acupuncture group,and the difference was significant(P < 0.01).Conclusion:1.acupuncture points "Jieyu prescription" can significantly improve the symptoms of depression in rats.2.in this experiment,the treatment of acupuncture points on the stagnation of the rat hippocampus weight,but there was no statistical significance.3.After stress stimulation,the neurons in hippocampus of model group were significantly reduced,while the number of neurons in hippocampus of acupuncture group was relatively less,Therefore,we may infer that acupuncture point "Jieyu prescription" can protect the hippocampus neurons in rats.4.after stress,the content of Ras protein in hippocampus of rats was increased,theexcessive activation of Ras-Erk pathway,excessive activation of acupuncture Jieyu Decoction can reduce the content of Ras protein in hippocampus of rats,inhibiting the Ras-Erk pathway in depression.To sum up,the mechanism of acupuncture treatment of depression may be related to the protection of hippocampal neurons,the reduction of Ras protein in hippocampus of depressed rats and the inhibition of Ras-Erk pathway. |
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