| Objective:Skin allergy means subject to the application of the classic model of HSP rat animal model,explore the effective parts of Qi Yuan granule(Group)effect and mechanism of treatment of allergic purpura,the Qi Yuan particles more widely used and provides theoretical and practical basis and clinical.If Qi Yuan particle effective parts(Group)of new drugs can be developed successfully,will further enhance the original clinical efficacy,for more patients to bring the gospel,promotion prospects are very broad.Method:Totally 80 male SD rats of clean grade,weight of 180±20g,aged 6 weeks.In a clean class animal room,the indoor temperature is maintained at(22±1)C,and the relative humidity is maintained(46±5)%.To feed a week later,on 80 SD rats were randomly divided into 8 groups,namely the normal group,model group,positive control group(hereinafter referred to as the cimetidine group,positive group),Yuan Qi granule group,volatile oil group,polysaccharide and protein,saponin group,flavonoids group group,10 rats in each group,each group before the experiment,free drinking water feeding.In addition to the normal group,the other groups were made model.With ginger,pepper,long pepper with 1: 1: 1 ratio in the constant temperature of 60 DEG ? water bath condensed into 12.5% /mL water solution,the 4?refrigerator storage backup.The animal experiment to adapt to the environment after 1 weeks except the normal group,the other 7groups of rats by gavage,3ml/singe,1 /d,normal group were given normal saline by gavage,free diet.Medication for 3 weeks.After the model group,positive group,Qi Yuan granule group,volatile oil group,polysaccharide and protein group,saponin group,Huang Tong group rats were given normal saline by intraperitoneal injection of 2mL/ containing ovalbumin 1mg + 0.5mL + 800 ng + LPS Aluminium Hydroxide Gel 1% Evans blue only,1times /7d,a total of 2 times.After the second injection of saline 1mL/ 10 min within the skin painted with OVA1 mg + Aluminium Hydroxide Gel 0.5mL + 800 ngLPS only induced allergic reaction.The normal group was treated with equal physiological saline.In allergized rats one week after treatment group respectively with Qi Yuan particles concentrated medicine(Astragalus 30 g,Xuanshen Decoction 20 g,angelica 15 g,honeysuckle 15 g,Rhizoma imperatae 9g,licorice 6g),polysaccharide and protein,total flavonoids,total saponins,total volatile oil by gavage,2ml/,1 /d,positive group with equalcimetidine treatment,for 14 consecutive days.The rat serum and urine protein pathological indicators to verify the model,the body weight of rats during the statistical and general behavior,after the intraperitoneal injection of 10% chloral hydrate anesthesia,abdominal aortic blood routine,centrifugal serum preparation to the standby-80 low temperature refrigerator,remove the kidney,with saline rinse clean and placed in formaldehyde fixed alternate in.After the preparation of samples,the ELISA technique was used to treat blood and urine samples,and the pathological changes of kidney were examined by light microscopy and electron microscopy.The data were processed by SPSS13.0,and the independent samples t test was used,and the difference was significant with p<0.05.Results:(1)there were significant behavioral changes in the animals during the experiment.The pathological indexes(CIC,IL-2,UP)of the model group were abnormal,which indicated that the model of the anaphylactoid purpura was good and could be used as an animal model.The volatile oil,polysaccharide,protein,saponin,flavone and so on can effectively regulate and control the biochemical indexes,and can effectively treat and treat allergic purpura.(2)ELISA results:?The normal group rats serum CIC concentration was lowest,the concentration of serum CIC in the rats of model group was highest,the difference was significantly(P<0.05);positive group,Qi Yuan granule group,polysaccharide and protein group,flavonoids group,saponin group,volatile oil group rats serum CIC concentration compared with the model group had obvious difference(P<0.05).Shows good therapeutic effect.The comparison between each treatment group,positive group and Qi tuple comparison,there were not obvious difference(P>0.05),and the difference between the positive group and Qi tuples with the other treatment groups significantly(P<0.05),but no significant difference compared with the normal group(P>0.05),shows the best effect of tuples in the positive group and treatment group compared with the polysaccharide;the other treatment groups had obvious difference(P<0.05);flavone group and other treatment group had obvious difference(P<0.05);comparison of volatile oil group and other treatment groups differed significantly(P<0.05),but no significant difference compared with the saponingroup(P>0.05),shows that volatile oil group and ginsenoside treatment level is relatively flat;and comparison of saponins the other treatment groups had obvious difference(P<0.05),but compared with the volatile oil group was not significantly different(P>0.05),indicating that the treatment effect of the two quite.?The normal group rats serum IL-2 concentration was lowest,the concentration of serum IL-2 in the rats of model group was highest,the difference was significantly(P<0.05);positive group,Qi Yuan granule group,polysaccharide and protein group,flavonoids group,saponin group,volatile oil group rats serum IL-2 concentration significantly difference compared with the model group(P<0.05)that shows good therapeutic effect.The comparison between each treatment group,positive group and Qi tuple comparison,there were not obvious difference(P>0.05),and the difference between the positive group and Qi tuples with the other treatment groups significantly(P<0.05),but no significant difference compared with the normal group(P>0.05),shows the best effect of tuples in the positive group and treatment group compared with the polysaccharide;the other treatment groups had obvious difference(P<0.05);flavone group and other treatment group had obvious difference(P<0.05);comparison of volatile oil group and other treatment groups differed significantly(P<0.05),but no significant difference compared with the saponin group(P>0.05),shows that volatile oil group and ginsenoside treatment level is relatively flat;and comparison of saponins the other treatment groups had obvious difference(P<0.05),but compared with the volatile oil group was not significantly different(P>0.05),indicating that the treatment effect of the two quite.? The minimum number of UP rats in the normal group,rats in the model group the number of UP is highest,the difference was significantly(P<0.05);positive group,Qi Yuan granule group,polysaccharide and protein group,flavonoids group,saponin group,volatile oil group the number of UP rats compared with model group significantly(P<0.05),indicating that the treatment effect good.The comparison between each treatment group,positive group and Qi tuple comparison,there were not obvious difference(P>0.05),and the difference between the positive group and Qi tuples with the other treatment groups significantly(P<0.05),but no significant difference compared with the normal group(P>0.05),shows the best effect of tuples in the positive group and treatment group compared with the polysaccharide;the other treatmentgroups had obvious difference(P<0.05);flavone group and other treatment group had obvious difference(P<0.05);comparison of volatile oil group and other treatment groups differed significantly(P<0.05),but no significant difference compared with the saponin group(P>0.05),shows that volatile oil group and ginsenoside treatment level is relatively flat;and comparison of saponins the other treatment groups had obvious difference(P<0.05),but compared with the volatile oil group was not significantly different(P>0.05),indicating that the treatment effect of the two quite.(3)renal pathology Normal kidney tissue showed no abnormal changes were observed in the model group,glomerular atrophy,interstitial hyperplasia,small vascular dilatation and congestion,renal tubular structure disorders;Kidney Qi tuples visible glomerular and renal tubular no obvious abnormal change,micro inflammatory cell infiltration;renal tubular tissue positive group had no obvious change,a small increase in glomerular number trace,inflammatory cell infiltration;polysaccharide group,flavonoids group showed glomerular number,interstitial vascular dilatation,less renal inflammatory cell infiltration;saponin group,volatile oil group glomerular number,interstitial,dilation of blood vessels,renal tubule tubule swelling,hemorrhage,inflammatory cell infiltration.Conclusion:(1)Qi Yuan compound granule and effective extract can reduce 24 h urinary protein quantitative experimental rat model,reduce the experimental rat model of circulating immune complexes(CIC)content,lower IL-2 content in serum of rat model,reduce the experimental model of rat mesangial IgA,IgG,IgAIgG deposition,improve renal blood vessels endothelial.(2)HSP,total polysaccharide,protein,total flavonoids,total saponins and total volatile oil were all able to interfere with the therapeutic effect.According to the results of the data analysis,it is shown that the curative effect is the total volatile oil,the total polysaccharides and total flavonoids(total saponins).(3)the HSP model established in this study,which is based on the establishment of the allergic constitution,the continuous antigen stimulation and the allergic reaction induced by antigen shock,can be repeated,and can be used as an animal model of HSP... |
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