Expression Of CMIP And MDM2 In Hepatocellular Carcinoma And Their Clinical Significance

Background: Hepatocellular carcinoma(HCC)is one of the major types of primary liver cancer.In our country,HCC is one of the major cancers leading to human death,which seriously endangers human health.Up to now,no breakthrough has been made in the treatment of HCC.The infinite growth of tumor cells is the result of inhibition of tumor cell apoptosis,and apoptosis is closely related to the occurrence and development of tumors.Studies have shown that c-maf inducing protein(CMIP)and murine dual-microgene 2(MDM2)are involved in the process of apoptosis,but whether these two genes play interaction through apoptosis-related pathways during the development of HCC is still uclear.Therefore,we explore the roles of these two genes in the development and progression of HCC from the protein and m RNA levels,and discuss the correlation between the two proteins.In order to provide theoretical basis for studying the mechanism of occurrence and development of HCC.Objective: To observe the expression of c-maf inducing protein(CMIP)and murine double minute 2(MDM2)protein in hepatocellular carcinoma(HCC)and adjacent tissue.Explore the clinical role and clinicopathological significance of CMIP and MDM2 in the development and progression of HCC.Methods: The expression of CMIP and MDM2 protein in 115 cases of HCC and 98 cases of corresponding adjacent tissues were detected by immunohistochemical staining.The expression of CMIP in 26 surgically resected fresh HCC and its adjacent tissues was detected by reverse transcription quantitative PCR(RT-q PCR)and Western blot.Finally,the relationship between CMIP/MDM2 and clinicalpathological parameters of HCC were analyzed,and the correlation between CMIP and MDM2 expression were also explored.Results: 1.The expression of CMIP located in the cytoplasm of HCC and adjacent tissues,and the positive cells were flaky distribution.The positive expression rate in adjacent tissues was 100.00%(98/98),which was significantly higher than that in HCC tissues 57.39%(66/115)(P<0.001).2.The positive staining of MDM2 protein in the cytoplasm of HCC and paracancerous tissues showed brown granules and flake distribution.The positive expression rate of MDM2 protein in the adjacent tissues was 12.24%(12/98),significantly lower than that in HCC tissues 57.39%(66/115)(P<0.001).3.The results of RT-q PCR showed that the expression of CMIP m RNA in fresh HCC tissues was significantly lower than that in paracancerous tissues,and the difference was statistically significant(P <0.05).4.The results of Western blot showed that the expression of CMIP protein in fresh HCC tissues was significantly lower than that in paracancerous tissues,the difference was statistically significant(P <0.001).5.The expression of CMIP protein in HCC tissues was closely related to tumor size(P<0.05)regardless of gender,age,hepatitis B surface antigen(HBs Ag)status,presence or absence of vascular invasion,alpha-fetoprotein(AFP)levels,liver capsule invasion,histological grade and TNM staging(P?0.05).6.The expression of MDM2 protein in HCC tissues was not correlated with gender,age,tumor size,HBs Ag status,presence or absence of vascular invasion,AFP levels,liver capsule invasion,histological grade,and TNM staging(P?0.05).7.There were no significant correlation between CMIP and MDM2 protein expression in HCC(r=-0.067,P=0.478).Conclusion: There are a high proportion of CMIP which lack of expression and MDM2 overexpression in HCC.The inactivation of CMIP and activation of MDM2 play an important role in the occurrence of HCC.