The Expression Of WWOX Gene In Osteosarcoma And Its Clinical Prognostic Relevance

ObjectionTo study the expression level of the wwox(ww domain containing oxidoreductase,wwox)gene in osteosarcoma and osteochondroma tissues,and to analyze its relationship with clinically relevant characteristics and prognosis.To explore the relationship between the expression of WWOX gene and the biological behavior of osteosarcoma,such as the occurrence,development and metastasis,so as to provide a theoretical basis for the prevention and treatment for osteosarcoma.MethodsA total of 106 paraffin-embedded specimens of osteosarcoma confirmed by pathology from January 2011 to December 2013 were selected from the First Affiliated Hospital of Zhengzhou University.The follow-up period was from February to 60 months,with an average of 42 months.Twenty patients with osteochondroma of the same period were selected as controls.The expression of WWOX protein encoded by these two groups of tissues was detected by streptavidin.perosidase(SP)method.The relationship between WWOX gene expression and patient age,gender,tumor size,distant metastasis,and clinical prognosis was analyzed.Statistical analysis using SPSS 21 statistical software analysis,two tests between the two groups,Kaplan-Meier survival analysis,P <0.05 was considered statistically significant.Results1.Expression of WWOX in osteosarcoma: A total of 106 patients with osteosarcoma were included in this study and 20 osteochondromas were used as controls.According to the results of immunohistochemistry,WWOX was divided into positive group and negative group.Immunohistochemistry results showed that WWOX was positively expressed in osteosarcoma and the positive reaction was mainly cytoplasm.The positive rate of WWOX expression in osteosarcoma group(47.2%)was lower than osteochondroma group(75.0%),the difference was statistically significant(P<0.05).2.Relationship between WWOX and clinical prognosis of patients with osteosarcoma:The distant metastasis(24.0%)in the positive group of WWOX gene expression in the observation group was significantly lower than that in the negative control group(57.1%).The 3-year survival rate(80.0%)and 5-year survival rate(72.0%)in the positive group were significantly higher than the 3-year survival rate(60.7%)and 5-year survival rate(48.2%)in the negative group.The difference was statistically significant.(p<0.05).The maximum diameter(>5cm)(50%)in the positive group was lower than the maximum diameter(>5cm)in the negative control group(53.6%),and the difference was not statistically significant(p>0.05).There was no significant difference with the age and sex of the patient(p>0.05).3.Kaplan-Meier method was used for survival analysis.By analyzing the survival time of follow-up patients,the survival time of WWOX positive group was higher than that of WWOX negative group,and the difference was statistically significant(p<0.05).Conclusion:The expression of WWOX gene encoded protein is significantly correlated with the occurrence and development of osteosarcoma.The occurrence and progression of osteosarcoma can significantly reduce the expression of WWOX gene-encoded protein.Therefore,WWOX gene can be used as one of the effective indicators to predict the recurrence of osteosarcoma and it may become an effective target for gene therapy in the future.