Expression And Signiffiance Of PTEN PI3K And AKT Proteins In Endometrial Polyps

Background Endometrial polyps(EP)is one of the most common gynecological benign diseases,mainly characterized by abnormal uterine bleeding and infertility.The gold standard for clinical treatment of EP is hysteroscopic endometrial polypectomy.In recent years,it has been studied that surgery combined with drug treatment can effectively prevent recurrence,and drug treatment needs to be started from the pathogenesis.The mechanism of EP has not been clear yet.In recent years,the study of signaling pathways involved in abnormal cell proliferation has become a hot topic.It is well known that phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway plays an important regulatory role in the regulation of cell growth,proliferation,anddifferentiation.Thephosphataseandtensinhomologue deletedcllrommosom10(PTEN)is a tumor suppressor gene with phosphatase activity and is elevated to the endometrium in various endometrial diseases.The height of the gene.Studies have shown that PTEN,PI3K,and AKT genes play an important role in cell proliferation,differentiation,and anti-apoptosis.However,whether the PTEN,PI3K,and AKT genes participate in the formation of EP has not yet been clarified..Objective In this study,immunohistochemical SP method was used to detect the expression of PTEN,PI3K and AKT protein in EP,analyze the relationship between them,and explore its significance in the pathogenesis of EP,and provide the basis for clinical treatment and prevention of EP recurrence.Methods From October 2016 to March 2017,we performed hysteroscopic EP enucleation in the planned ward in the First Affiliated Hospital of Guangxi Medical University.postoperative pathology confirmed 30 patients with EP(EP group);there was no abnormal endometrial tissue around 0.5cm to1.0cm in the peripheral part of the polyp,25 cases of intimal tissue around the polyp(EP surrounding group);in the control group,infertility patients underwent laparoscopic surgery for endometrial microstimulation to scrape the endometrium.Tissue,pathologically confirmed proliferative endometrium in 25cases.The expression of PTEN,PI3K and AKT protein in EP group,EP group and control group were detected by immunohistochemical SP method.Using SPSS17.0 statistical software,the measurement data was expressed as(?x±s).The count data were analyzed using the?~2 test,multivariate analysis using one-way analysis of variance and the correlation was analyzed using the Spearman rank correlation analysis.P<0.05 for the difference was statistically significant.Result1.Expression of PTEN,PI3K and AKT Proteins in Each Group1.1 PTEN was mainly expressed in the endometrial glandular epithelial cytoplasm;the positive rates of PTEN in the EP group,the control group,and the EP group were 33%,72%,and 68%,respectively;the EP group was less positive than the control group and the EP group.(P<0.00),the difference was statistically significant.1.2 PI3K was mainly expressed in the endometrial glandular epithelial cytoplasm;the positive rates of PI3K in the EP group,the control group,and the EP group were 67.7%,36%,and 24%%,respectively;the positive expression rate of the EP group was higher than that of the control group and EP In the peripheral group(P<0.00),the difference was statistically significant.1.3 AKT is mainly expressed on the nuclei of endometrial glandular epithelial cells.The positive rates of AKT in the EP group,the control group,and the EP group were 83%,36%,and 24%,respectively.The EP group was more positive than the control group and the EP group.P<0.00),the difference was statistically significant.2.PTEN,PI3K,and AKT scores in each group2.1 PTEN scores were 1.495±0.557,2.220±0.508,and 2.038±0.393 in the EP group,the control group,and the EP group;PTEN scores were lower in the EP group than in the control group and the EP group(P<0.00),the difference was statistically significant.2.2 The scores of PI3K in the EP group,the control group,and the EP group were 1.965±0.468,1.548±0.578,and 1.458±0.452,respectively;PI3K scores in the EP group were higher than those in the control group and the EP group(P<0.00),the difference was statistically significant.2.3 The scores of AKT in the EP group,the control group,and the EP group were 2.097±0.485,1.678±0.564,and 1.396±0.485,respectively;the AKT scores in the EP group were higher than those in the control group and the EP group(P<0.00).The difference was statistically significant.3.Expression of PTEN,PI3K and AKT proteins in EP groupIn the EP group,the expression of AKT and PTEN was negatively correlated(r=-0.457,P<0.05),PI3K was negatively correlated with PTEN(r=-0.533,P<0.05),PI3K and AKT were positively correlated(r=0.405,P<0.05).Conclusion1.The expression of PTEN protein in EP group was significantly lower than that in control group and EP group.The expression of PI3K and AKT protein in EP group was significantly higher than that in control group and EP group.Presumably,there was low expression or absence of PTEN protein in EP formation.Accompanied by PI3K,AKT protein overexpression.2.In the EP group,the expression of AKT protein and PTEN protein was negatively correlated,PI3K protein was negatively correlated with PTEN protein,PI3K protein and AKT protein were positively correlated,indicating that the decrease of PTEN expression in the process of EP development and apoptosis Inhibition weakened,which stimulated the high expression of PI3K and AKT proteins to increase cell proliferation,which in turn led to excessive endometrial cell proliferation,decreased apoptosis,and promoted the continuous proliferation of the endometrium into EP.