| Objective:To establishment of mouse model of dyspepsia,studying on the effect of Tiaopi Chengqi Decoction on the gastric emptying and small intestine propulsion in experimental rat model of indigestion,serum gastrin(GAS),motilin(MTL),somatostatin(SS),vasoactive intestinal peptide(VIP);And the effect of Tiaopi Chengqi Decoction on gastric acid and pepsin secretion in rats was studied;And what effect it will have on commom intestinal and respiratory tract flora.Explore the mechanism of action of Tiaopi Chengqi Decoction on children indigestion and its bacteriostasis.providing definite theoretical foundation for the clinical applications of the medicine used on children indigestion.Methods:Through the high calorie and protein solution and fodder,the atropine is administered by intraperitoneal injection to build the animal model with dyspepsia.record general condition,weight and abdominal perimeter of animals every day,and detect the comment appetite in each phase of the animals.Measure the gastric emptying and small intestine propulsion after administration;The GAS,MTL,SS,VIP are determined by radioimmunoassay;Use pyloric ligation to collect tne gastric juice,and use titration and improve the Mett to observe the affection of the medicine to the gastric acid and pepsin secretion.To determine the inhibition zone diameters with Cup-plate method and observe the bacteriostasis in vitro and use the dilution separation method to observe the pre-enrichment of the Tiaopi Chengqi Decoction to the intestinal flora.Results:Contrasted with the model group,the high and middle does group of Tiaopi Chengqi Decoction is more effective in speeding up gastric empting and small intestinal propulsion of mice(P<0.01,P<0.05).The serum MTL and SS of mice are increased and the serum GAS and VIP of mice are decreased after the model is established.Serum GAS,MTL,SS and VIP levels has returned to nearly normal after giving medicine.And that Motilium group and high does group were more effective than the model group(P<0.05,P<0.01).Compared with the blank group,the high and middle does group of Tiaopi Chengqi Decoction could significantly promote the secretion of pepsin of rats(P<0.05,P<0.01),but it had no effect on the secretion of gastric acid(P>0.05).Tiaopi Chengqi Decoction of high,medium and low dose group can increase the activity of pepsin of rats,the high dose group was most obvious,compared with the blank control group,there was significant difference(P<0.01).The inhibitory zone diameter of all the tested bacteria is 0.And there is no obvious enriching effect on intestinal probiotics.Conclusion:Tiaopi Chengqi Decoction can promote intestinal peristalsis and gastric emptying,improve the content of motilin and gastrin in serum,decrease of vasoactive intestinal peptide and somatostatin levels,improve the activity of pepsin.The accumulation of heat accumulation in the spleen and stomach may be related to the above effects.But Tiaopi Chengqi Decoction is not sensitive to common gastrointestinal and respiratory tract pathogenic bacteria for common intestinal pathogenic bacteria,probiotics are part of the opportunity to not kill or promote.In other words,the Tiaopi Chengqi Decoction does not disturb the ecological balance of intestinal microflora in the treatment of diseases. |
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