| ObjectivesBiological sample detection data from around the world have shown that human exposure to bisphenol A(BPA)was widespread.BPA exposure during pregnancy could disrupt the normal neuroendocrine function,and increase the risk of growth and development disorders in children,especially the risk of neurodevelopmental disorders.At present,few of studies on the relationship between BPA exposure during pregnancy and neurobehavioral development in their offspring in China.Ample prospective birth cohort studies abroad have investigated the associations between maternal BPA exposure and neurobehavioral development in children.However,the findings are inconsistent.In view of this,the present study evaluated the BPA exposure level of the pregnant women and investigated the prevalence of emotional and behavioral problems of their children in the cohort,further explored the risk factors.The aim of present study was to investigate the relationship between maternal BPA exposure and risk of emotional and behavioral problems.MethodsThis study was based on China-Anhui Birth Cohort Study(C-ABCS).The study population were enrolled in C-ABCS between November 2008 and October 2010.The pregnant women meeting the eligibility criteria were enrolled into the cohort.A unified questionnaire was completed by the women independently at their first prenatal examinations.Maternal fasting blood samples during pregnancy were collected in the morning.Then maternal serum was obtained after 15 minutes of blood centrifugation.After collection,the samples were then inventoried and,kept in-80℃refrigerator,and shipped to the Anhui Provincial Key Laboratory of Population Health and Aristogenics for analysis.The serum free concentrations of BPA were measured using isotope dilution-solid extraction(SPE)coupled to high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).This research analyzed a subset of C-ABCS that recruited 5,084 eligible participants were from Ma’anshan city of Anhui province.After excluding pregnant women lost to follow up(n=202),spontaneous abortions(n=92),giving birth to twins(n=66),odinopoeia,fetal death and stillbirth(n=55),a total of 4,669 singleton live births had been followed up.The data of childhood executive function in present study was from the follow-up visits between April 2014 and April 2015,and 3,803 children were followed up.Between December 2012 and January 2014,the BPA concentrations of 1,783 pregnant women had measured BPA concentrations were measured.Except for 70 preschool children who did not have Strengths and Difficulties Questionnaire(SDQ)evaluation result among the 1,783 children,there were totally 1 713 mother-and-only-child pairs who were admitted to the present analysis finally included in the final analysis.The subjects were devided into low exposure group(≤0.120 ng/ml),middle exposure group(0.120<BPA<0.400 ng/ml)and high exposure group(≥0.400 ng/ml)according to the serum BPA concentration in tertile.We performedχ~2 analyses of association between categorical the emotional and behavioral problems dysplasia and serum free BPA concentrations tertiles.The association between BPA exposure during pregnancy and the emotional and behavioral problems in preschool children were evaluated with multinomial logistic regression model.ResultsAmong the serum samples of 1 713 pregnant women,1 014 serum samples were collected during first trimester and 699 serum samples were collected during second trimester of pregnancy.The concentrations of serum free BPA ranged from 0.001 to20.718 ng/mL.The detected rate of emotional problems in 1 713 preschool children was6.48%,8.11%for conduct problems,8.35%for hyperactivity/Inattention,2.86%for peer problems,11.38%for prosocial behaviors,7.94%for total difficulties.Multivariate logistic regression analysis showed that maternal BPA exposure during pregnancy in the high level was associated with the children’s conduct problems abnormalities(OR=1.876,95%CI:1.161~3.029).That is said that the risk of conduct problems abnormalities with the high level BPA exposure during pregnancy was 1.876times higher than that of the children whose mothers with the low level BPA exposure during pregnancy.After children’s gender stratification,the multivariate logistic regression analysis showed that maternal BPA exposure at high level was associated with the conduct problems abnormalities was modified by child gender and the association significant was seen among boys(OR=2.291,95%CI:1.126~4.661).ConclusionMaternal exposure to the high level of BPA during pregnancy may increase the detrimental effects of conduct problems abnormalities in preschool children,and it was more obvious among boys.Due to the limitations brought by the maternal chronic BPA exposure during pregnancy estimated based on a single serum sample,the potential risk factors impact on the child neurobehavioral development and other confounders,it is necessary that to determine the sensitive period of BPA exposure during pregnancy by extra studies of epidemiology and animal experiments.It would contribute to reveal the specific biological mechanisms related to BPA exposure during pregnancy increase the detrimental effects of neurobehavioral development in the offspring. |
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