Molecular Epidemiology Study Of Mras Gene Polymorphism And Essential Hypertension

Objective: A new cardiovascular disease risk gene called the Mras gene was discovered through the GWAS study in European.To propose theoretical basis for exploring the pathogenesis of essential hypertension(EH)and finding new dangerous genes,the study would investigate the genetic effect of Mras gene(rs40593,rs751357 and rs6782181)on the susceptibility of EH in the population of north Anhui.Methods: 643 EH patients and 548 controls in People's Hospital of north Anhui Province were enrolled as subjects whose age was between 35-75.Meanwhile,the questionnaires were used to collect the baseline data of general demographic data,disease history data,lifestyle behavior data,physical examination data and biochemical index data of the case group and the control group.In this study,DNA was extracted by protein precipitation and the polymorphism of Mras was detected by Taq Man genotyping.Logistic regression,general linear model and other statistical methods were used to analyze the gene and disease association.Result: There was no significant differences between sex,TC,LDL-C,smoking and drinking in the hypertension group and control group(P>0.05).The age,TG,HDL-C,GLU and BMI in the hypertension group were higher than those in the control group,but the control group had higher HDL-C(P<0.01).There were no significant differences in genotype and allele frequency distribution between rs40593,rs751357 and rs6782181 in hypertension group and control group(P > 0.05);Association analysis showed that rs751357 and rs6782181 were not associated with hypertension in the additive,dominant,and recessive models,but the risk of hypertension in the GG genotype at rs40593 was 1.665 times that of the(AA+AG)genotype,P=0.038.After further correcting the relevant covariates,the association was not statistically significant.Subgroup analysis showed that after correcting the variables the female population and non-drinking population of rs40593 was associated with hypertension in the additive model,dominant model,and recessive model(P<0.05).The rs751357 locus was associated with hypertension in the female population in the additive model [1.423(1.057-1.916),P=0.020] and the dominant model [1.492(1.033-2.154),P=0.033].In the non-drinking population,rhe rs751357 was associated with hypertension in addition models,dominant models,and recessive models associated with hypertension.Similarly,in the non-drinking population,rhe rs751357 was associated with hypertension in addition models [1.377(1.070-1.772),P=0.013] and recessive models [2.314(1.235-4.338),P=0.009].There was no significant difference in systolic blood pressure and diastolic blood pressure with rs40593,rs751357,and rs6782181 different genotypes between hypertensive medication group,hypertensive untreated group and non-hypertension group(P>0.05).Conclusion: Age,TG,HDL-C,GLU,and BMI were risk factors for hypertension in northern Anhui.There was no association between the polymorphism of the Mrs gene and hypertension in the population.Stratified analysis revealed that the rs40593 and rs751357 of the Mras gene might have a gender-specific impact on the hypertension.The gene mutation might be a risk factor for hypertension in the female population;Mars gene rs40593,rs751357 and rs6782181 were associated with the risk of hypertension in non-drinking people and the gene mutation might be a risk factor for hypertension in non-drinkers.There was no significant difference in systolic blood pressure and diastolic blood pressure with rs40593,rs751357,and rs6782181 different genotypes between hypertensive medication group,hypertensive untreated group and non-hypertension group(P>0.05).