Hui Yang Gu Yuan Fang Righting Effect On Apoptosis Of Human Papillomavirus Infection

human papillomavirus is a small molecule of double-stranded DNA virus,human skin and mucous membrane with a high degree of tropism.According polymorphic gene sequence,has been found more than 200 kinds of clinical subtypes,can cause common warts,flat warts,toe warts and Condyloma acuminatum,and other skin diseases,of which the most harmful warts,but also our research focus.Is a Condyloma acuminatum from human papillomavirus(HPV)caused by sexually transmitted diseases,the incidence rate is still increasing year by year,ranking third in the country for sexually transmitted diseases,a higher incidence in Europe and the United States,in recent years,to receive widespread attention.At present,the pathogenesis of genital warts is not yet clear,studies have shown that it may be related to the cell cycle.HPV infection of skin and mucous membrane infections caused by a benign proliferative disease,clinical treatment more difficult,the recurrence rate is higher because there are different degrees of subclinical and latent infection,and some may also be cancerous,leading to cervical cancer.Currently,often using immunomodulatory therapy clinic,however,different patients respond to immunotherapy there are individual differences.Genital warts is growing at a huge mass destruction threat to people's health.HPV-infected cells from the chill yang thing,righting the main treatment is mainly to warm yang,yang and the characteristics of the drug is not clear intense cells,while gently mutagenic cells.For this reason,the use of Caco-2 monolayer cell culture method for modeling serum pharmacology method of administration,the use of induced acclimation to HPV-infected cells Yunnan characteristics of effective solid element back to Yang Fang Onyang drugs,thereby changing its cells yin and yang state,prompting state yin and yang equilibrium state.Yuan Fang and Yang solid back by the drink evolved back to the sun,drink back to the Qing Dynasty in Shenyang doctors Zheng Qinan treatment roadway losses set as a basis,the late famous Yunnan Province Onyang everyone "Wu Fu Zi" Mr.Wu Peiheng Huiyang create large drink in a variety of Chinese and Western medicine clinical disease refractory to quite good effect,the party containing aconite,ginger,aconite as the first drug Onyang,Onyang well-known people,"Wu Fu Zi" Mr.Wu Rongzu continue the development of solid yuan Fang Hui Yang,including genital warts,including the efficacy of intractable diseases has been further improved.In this study,we used different concentrations of solid Yuan Fang Hui Yang,After a Transwell conversion pool absorption,skin lesions and genital warts in patients extracted keratinocytes-after a single cell suspension and the suspension of normal keratinocytes role,with a flow cytometry determined the extent of cell cycle and apoptotic cells by RT-PCR(RT-PCR)in its cell ?-catenin mRNA ?-catenin)expression were detected to observe yang solid yuan Fang HPV infection of the biological characteristics of the cells.The results showed that patients with genital warts by reverse transcription polymerase chain(RT-PCR)method for detection of lesions keratinocytes ?-catenin mRNA ?-catenin)expression level was significantly higher,indicating that warts pathogenesis of certain proteins that may arise over the case expression.Periodic HPV-infected cells by flow cytometry and cell and molecular biology Onyang drugs after excitation,apoptosis,cell proliferation have different degrees of change,biological properties of the cell has undergone some changes,the traditional Chinese medicine after the degree of cell density effect is small,round cells towards the changing trends,which significantly accelerate the cell cycle,proliferation and diminished capacity,the degree of apoptosis has also undergone some changes.Thus,we find the angle cytology Chinese medicine-induced apoptosis and HPV-infected cells to their cell cycle.In summary,we use righting the role of the male solid element back side forming cells induce a certain extent domesticated Lesions horny,yang solid Yuan Fang shows the potential value of a new drug treatment of genital warts,which has high academic value,but also provide valuable research results for the development and application of Yunnan local medicine.