Preparation Of Curcumin Liposomes And Study Of Its Antibacterial Activity In Vivo And In Vitro

Staphylococcus aureus is the main pathogenic bacteria in animal clinics,which can cause various inflammatory reactions,such as cow mastitis,pneumonia,pericarditis,etc.Antibiotics are specific drugs to inhibit Staphylococcus aureus,but in the treatment of bacterial infections,The abuse of antibiotics has made the resistance of bacteria more and more serious,and curcumin has strong antibacterial activity,especially against Staphylococcus aureus,Because curcumin is not easily soluble in water,it cannot be promoted and applied clinically,This test change the curcumin dosage form,wrap curcumin in phospholipids,increase the solubility of curcumin,and further explore the antibacterial activity of curcumin liposomes.Firstly,an analysis method in vitro for curcumin was established.The UVspectrophotometric method can be used to detect the drug content in curcumin liposomes.This method is simple to operate and has good reproducibility.Secondly,the curcumin liposomes were prepared by ethanol injection,and the encapsulation rate was used to investigate the single factor to determine the influencing factors;the Box-Behnken Design response surface analysis method was used to further optimize the curcumin lipid based on the encapsulation rate.The optimal prescription is determined as follows: phospholipid to cholesterol is 5:1,drug-to-lipid ratio is 1:50,buffer p H is 7.4,Na Cl content is 29.89 mmol/L,and the encapsulation rate is 92.7%;The results of the properties test showed that the average particle size of curcumin liposomes was 152.7 nm,and the Zeta potential was-2.00 m V;curcumin liposomes were released in PBS containing 0.1% Tween,and the release was faster within 12 h,and after 12 h The release tends to be flat,with a 48-hour release rate of 90.252%.When stored at 4.0°C for 60 days,the encapsulation rate decreases slightly.When stored at 25°C,the encapsulation rate decreases significantly,indicating poor stability of curcumin liposomes.It can be further lyophilized to improve its stability and extend storage time.Finally,the Pand in vitro antibacterial activity of curcumin liposomes was studied.In vitro antibacterial activity test results showed that curcumin liposomes have strong antibacterial activity against Staphylococcus aureus and the minimum inhibitory concentration against Staphylococcus aureus ATCC 29213(MIC)and minimum bactericidal concentration(MBC)were 32 ?g/m L and 128 ?g/m L,respectively.The survival of Staphylococcus aureus ATCC 29213 before and after the action of curcumin liposomes was further observed by laser confocal.The results showed that,When curcumin liposomes acted on the strain for 12 hours,a large number of strains died,indicating that curcumin liposomes had good antibacterial activity in vitro.The results of in vivo antibacterial assays showed that for mice infected with Staphylococcus aureus ATCC 29213,the number of bacteria in the ascites of the curcumin liposome group was significantly different from that of the blank control group.The curcumin liposome group was compared with free turmeric.Compared with the curcumin group,the number of bacteria in the ascites was extremely different;the curcumin liposome group was significantly different from the saline group in the survival rate of mice,and compared with the free curcumin group,the survival rate of the mice was extremely different,indicating that curcumin Liposomes have good antibacterial activity in the body.Compared with free curcumin,curcumin liposomes have better antibacterial effect on Staphylococcus aureus ATCC 29213 in vivo.