Effects Of Acanthopanax Senticosus Polysaccharides On Intestinal Mucosal Injury In Immune Stress Weaned Piglets

Early weaning of piglets is a common measure to improve economic efficiency in pig production.However,early-weaned piglets have not yet fully developed immune function and are susceptible to immune stress caused by environmental pathogens.Immune stress can cause damage to the morphology and function of the piglet’s intestinal mucosa,which not only causes the piglet’s feed intake and feed utilization to decrease,but also causes piglet diarrhea,and even leads to piglet growth retardation and death.The use of nutrition regulation is an important measure to relieve immune stress.This experiment adopts a single factor design and uses lipopolysaccharide(LPS)to stimulate a piglet immune stress model to study the effect of adding Acanthopanax senticosus polysaccharides(ASPS)in the diet on the intestinal mucosal damage of weaned piglets stimulated by LPS.Application of ASPS to improve piglet intestinal mucosal injury provides a theoretical basis.36 weaned piglets were randomly divided into 3 groups:(1)basal diet(control group);(2)basal diet + LPS stimulation(LPS group);(3)add 800 mg/kg ASPS basal diet +LPS stimulation(ASPS+LPS group).4 replicates per group,3 piglets in each replicate.The test period was 21 days,and each piglet was injected intraperitoneally with 100 ug/kg LPS on the15,18,and 21 days.On the 21 st day of the test,blood samples were collected and piglets were slaughtered.The small intestine,heart,liver and other internal organs and ileal mucosa were collected for the analysis of various indicators of the test.The results of the experiment found that compared with the LPS group,the addition of ASPS to the diet significantly reduced the number of leukocytes,neutrophils and monocytes in the peripheral blood of weaned piglets(P<0.05),and significantly increased the D-xylose in piglets’ plasma Content(P<0.05)and lactase content in intestinal mucosa(P<0.05).ASPS significantly improved the morphological deterioration of piglet intestinal villi height(P<0.05)and crypt depth(P<0.05)caused by LPS stimulation.At the same time,adding ASPS to the diet can significantly reduce the number of mast cells in the intestinal mucosa of piglets(P<0.05).The content of tumor necrosis factor(TNF-α)(P<0.05)and interleukin 1β(IL-1β)(P<0.05)of piglet intestinal mucosa was significantly decreased,and the content of interleukin 10(IL-10)in intestinal mucosa was significantly increased High(P<0.05),but the content of interleukin-8(IL-8)and interleukin-6(IL-6)in the intestinal mucosa did not change significantly(P>0.05).In addition,adding ASPS to the diet can significantly down-regulate the intestinal mucosal tumor necrosis factor(TNF-α)and hypoxia-inducible factor-1α(HIF-1α)and inducible nitric oxide synthase(i NOS)caused by LPS stimulation.Increase in gene expression(P<0.05).At the same time,it inhibited the increase of HIF-1α,cyclooxygenase-2(COX-2)and nuclear factor p65(NFκB p65)protein expression in the ileal mucosa stimulated by LPS(P<0.05).The results showed that the addition of ASPS to the diet can significantly alleviate the damage to the morphology and function of the intestinal mucosa of weaned piglets caused by LPS stimulation,and reduce the inflammation of the intestinal mucosa.This effect may be related to the signaling pathway of inflammation and hypoxia initiated by ASPS in regulating intestinal mucosa NFκB p65 and HIF-1α.