Effects Of Mineral Nutrirnts On Amaranth Growth And Functional Validation Of Its Zn Transporter

Amaranth is a vegetable of amaranth family.The thesis studies effects of different Ca²⁺、Mg²⁺、Zn²⁺、Fe²⁺、Mn²⁺concentration of on the growth of Amaranth seedlings.Meantime,based on bioinformatics analysis,eight Zn transporter-encoding genes were cloned and their Zn transportation activity was confirmed with yeast mutant system.Experimental results are followed:（1）It was studied of effects of Ca²⁺、Mg²⁺、Zn²⁺、Fe²⁺、Mn²⁺at different concentration on root and hypocotyl length,fresh weight and root hair growth.Under both pH 5.7 and 7.2,Ca²⁺deficiency and Fe²⁺oversupply inhibited the growth.Other mineral ions had no significant effects on it.（2）Increasing concentration of Fe²⁺could partially alleviate the inhibitory effect of Ca²⁺deficiency on the seedlings’growth.Increasing concentration of Ca²⁺could alleviate inhibitory effect of Cd,but not the high Fe²⁺on the growth.The growth of Amaranth seedlings was insensitive to the concentration of Zn²⁺and Zn²⁺had no effect on the inhibitory effect of high Fe²⁺.（3）Transcriptome analysis with the seedling’s RNA identified eight intact Zn²⁺transporter encoding genes named as:AmZTP29、AmZIP11、AmZIP2、AmZIP4、AmZIP1.1、AmZIP1.2、AmZIP1.3、AmZIP7.Among them,AmZIP4、AmZIP1.1、AmZIP1.2、AmZIP1.3 and AmZIP7 could complement the growth of the Zn²⁺transporter-deficit yeast on the low medium.