Breeding Of Excellent Lentinula Edodes Strains

China is not only a big country for producing mushrooms,but also a big country for consumption of Lentinula edodes.With the continuous improvement of people's living standards,people's quality requirements for Lentinula edodes are getting higher and higher.Traditional cultivation models and production varieties are far from meeting the needs of the market.Therefore,it is extremely urgent to breed high-quality new varieties of Lentinula edodes.In this study,Lentinula edodes varieties with different characteristics and excellent wild strains were selected as parents,and 2509 hybrid strains were obtained by mononuclear strains hybriding with mononuclear strains and mononuclear strains hybriding with binuclear strains.According to the multi-indicators of mycelial growth and agronomic traits,13 excelent hybrid strains were selected.The main findings are as follows:(1)Isolation and screening of mononuclear strains724 mononuclear strains were isolated from 13 parents' monospores.Mononuclear strains were excellented using four indicators: mycelial growth rates,colony types,colony growth scores and growth indexes,then 187 excellent mononuclear strains were screened.(2)Lentinula edodes hybridization and primary screeningBased on the characteristics of the parents,21 hybrid schemes were designed,and then 2509 hybrid strains were obtained.According to the growth rate,colony type,growth index,germination period and feeding time of the hybrid strains,750 excellent hybrid strains were screened.(3)Determination of agronomic traits of Lentinula edodes strainsA total of 1057 strains of the selected 750 hybrid strains and the mutagenized strains in the laboratory were cultivated.The growth of mycelium,the formation time and temperature of fruiting,the yield per bag,the number of mushrooms,the quality of single mushroom,the characteristics of mushroom shape and hardness were determined,and 13 excellent strains with early fruiting,high yield,good mushroom shape and high hardness were selected.(4)Preliminary identification of excellent strainsAfter 13 antagonistic experiments and esterase isoenzyme electrophoresis studies,13 excelent strains selected in this study were excellent hybrid strains.