Study On Sparse Planting Techniques Of Vigoroos Seedlings Of Coptidis Chinensis And Its Primary Processing Drying Method

The Coptis chinensis Franch as a research object in this experiment,and aim to its practical problems in cultivation and processing in the area of origin,we put the priority on the study of seedlings standard,sparse planting techniques of vigorous seedlings and drying methods in the area of origin.By experimental verification,like the investigation of accumulation of whole plant biomass,the content of soluble sugar,the total content of nitrogen,phosphorus,potassium in the different parts of C. chinensis plant,and the measurement of dynamic change in the content of total alkaloid,epiberberine,coptisine,palmatine,berberine,soluble alcohol extract,and ash.we established a appropriate method and the corresponding standard method of seedlings classification.Then discussed the physiological basis of spares planting of“vigorous seedlings”through the different density planting experiment.We have studied and evaluated the primary drying methods of fresh C. chinensis rhizome.The main results of this study are as follows:1. The height and number of leaves of C. chinensis presented normal distribution in its population,so the stepwise clustering as an efficient and straightforward classification method that was used to classify the C. chinensis seedlings.According to this method,the seedlings of C. chinensis divided into 3 levels.The classification standards are(1)First-level seedlings:plant height≥8.3cm,leaf number≥9;(2)second-level seedlings:4.9cm≤Plant height<8.3cm,5 pieces≤number of leaves<9;(3)Tertiary seedlings:plant height≤4.9cm,number of leaves<5.After one month of transplanting,the survival rate calculation showed that the survival rate of the first to third seedlings showed a decreasing trend.After 6 months of transplanting,we have found that the agronomic traits and active components of C. chinensis showed significant advantages in the first level,compared with the third-level seedlings.In addition,compared to the seedlings in the time of transplanting,the relative rate of biomass was increased 20.72%,13.80%,5.02%of the first,second,third grade seedlings respectively,these three classes were significantly different,these results suggested that the method of classification,affected the growth of different classes of C. chinensis seedlings.2. Different planting density treatments(30,000 plants/667 m~2,50,000 plants/667m~2,70,000 plants/667 m~2,90,000 plants/667 m~2were designed in this experiment)significantly affected the number of leaves,plant height,total plant biomass of C. chinensis,the agronomic traits(growth status,dry matter accumulation,root-shoot ratio,leaf area index)and photosynthetic characteristics,the content of total alkaloids,epiberberine,coptisine,palmatine,berberine of C. chinensis in different periods.Rare-planting beneficial to the early development of C. chinensis seedlings,the plants were more vigorous,and photosynthesis was developed.interestingly,the biomass of C. chinensis plants was larger under the rare plant density during the same growth period.The relationship of dry matter mass of rhizome under different density treatments was30,000 plants/667 m~2>50,000 plants/667 m~2>70,000 plants/667 m~2>90,000plants/667 m~2respectively.The content of soluble sugar,nitrogen,phosphorus,potassium,and other nutrients,total alkaloids,and the content of the four main alkaloids were 30,000 plants/667 m~2>50,000 plants/667 m~2>70,000 plants/667 m~2>90,000plants/667 m~2 respectively.Considering the early seedlings development,the individual plant quality traits,and the accumulation of alkaloid content,the density treatment of30,000 plants/667m~2 was the best.From the perspective of population yield,the control group was the best.Therefore,taking into account the yield and quality of C. chinensis were reasonably reduced by the planting density.3. Drying method at 4 different temperatures,such as 60℃、80℃、100℃、120℃.At 60℃,the total content of the medicinal components,and ash were the lowest,the content of total alkaloids,soluble alcohol extracts,epiberberine,berberine,palmatine,berberine were the highest.Among the three drying methods:sunlight drying,high-power electrothermal drying,and drum open flame drying methods,the sunlight drying and high-power electrothermal drying methods were better in each quality evaluation index.In addition,the drum open flame drying method can produce obvious charred dried rhizome dry samples.After testing,the alcohol-soluble extracts,epiberberine,berberine,palmatine,berberine,and other quality indicators have failed to meet the pharmacopoeia needs.The samples obtained by sunlight drying and electrothermal drying all meet the needs of the Pharmacopoeia.Therefore,based on this study,high-power electrothermal drying is the most suitable method for drying large-scale fresh rhizome of C. chinensis.All these results may provide scientific references for quality classification of C. chinensis seedlings,a reasonable cultivation density,and a better method of drying fresh rhizome in the place of origin.