Establish Rapid Propagation System Of Amygdalus Triloba Linbl.Ricker

The Amygdalus triloba Linbl.Ricker is a high-quality tree species for garden greening in northern China.Its dense foliage and colorful flowers are a new breakthrough in the garden color tree species.At present,traditional asexual reproduction techniques such as cutting and grafting have long production cycles and cannot meet the needs of urban greening in terms of output.And rapid propagation through tissue culture to meet production needs.In this experiment,the effective rapid propagation system of Amygdalus triloba Linb1.Ricker was established through the study of five stages,disinfection method of the current stem segment,induction and proliferation culture of clustered buds,callus induction and differentiation culture of stem segment,rooting culture and seedling refining.The results are as follows:1.The best sterilization method of the red stem of the Amygdalus triloba Linb1.Ricker in the year was to sterilize it with 75%alcohol for 30 s and then sterilize it with 3%sodium hypochlorite for 12 min.At this time,the explant pollution rate was 18.96%,and the survival rate was as high as 77.71%.2.In the cultivation of cluster buds induced by the stem segments of Amygdalus triloba Linbl.Ricker,MS is the best basic medium;the optimal hormone combination for cluster buds induced by the stem segments is:6-BA 1.5 mg/L+NAA 0.3 mg/L.The induction rate was 91.33%;the optimal hormone combination for cluster bud proliferation was:6-BA 1.5 mg/L+NAA 0.1 mg/L,and the proliferation coefficient was 11.07.3.In the callus induction culture of the stem of the Amygdalus triloba Linb1.Ricker,light culture is more conducive to the formation of callus;MS is the best basic medium;the best hormone combination for callus induction is 6-BA 1.0 mg/L+2,4-D 0.5 mg/L,the induction rate is 86.41%;the best hormone combination for callus proliferation is:6-BA 1.5 mg/L+IBA 0.4 mg/L,the proliferation rate is 78.35%;The best hormone combination for callus differentiation was:6-BA 1.5 mg/L+NAA 0.2 mg/L,the differentiation rate was 86.72%,and the average number of differentiated buds was 8.4.The best medium for rooting of aseptic seedlings of Amygdalus triloba Linb1.Ricker is 1/2MS+IB A 0.3mg/L,the rooting rate is 94.33%,and the average root length can reach 6.34 cm.5.When the Amygdalus triloba Linb1.Ricker tissue culture seedlings were refined,first open the bottle and refine the seedlings for 1 d to gradually adapt the tissue culture seedlings to the external environment,and then transplant the tissue culture seedlings with an average root length of 3 to 5 cm to the Nutrient soil:Vermiculite:Perlite=3:2:1 The survival rate on the seedling substrate was the highest,which was 82.33%.6.Comparing the two cultivation methods in this experiment,induction of cluster buds in current year's stem segments is the best way to establish a rapid propagation system of Amygdalus triloba Linb1.Ricker.