Effects Of Exogenous Hormones On Flower Bud Differentiati On And Fruit Setting Of Camellia Oleifera

In order to explore the effects of exogenous 6-BA and GA₃on flower bud differentiation and physiological and biochemical characteristics of Camellia oleifera,the dynamic changes of nutrient content in leaves during flower bud differentiation were analyzed,the correlation between the content of endogenous hormones and the rate of flower bud differentiation in flower buds during flower bud differentiation and the correlation between endogenous hormone content and fruit setting rate in Camellia oleifera are abserved,which can provide theoretical basis and technical guidance for improving the flower bud differentiation rate and fruit setting rate of Camellia oleifera in actual production.In this study,9-year-old Camellia oleifera excellent clonal’Changlin 4’was used as test material,and 0,100,200,300,400,500mg/L exogenous 6-BA and GA₃were sprayed separately,dynamic changes of endogenous hormones（IAA,GA₃,ZR,ABA）of hermaphrodite and leaf nutrient levels（soluble sugar,soluble protein）during flower bud differentiation of Camellia oleifera were analyzed to explore the effects of exogenous 6-BA and GA₃on flower bud differentiation and fruit setting of Camellia oleifera.The research results are as follows:（1）Exogenous 6-BA treatment can promote the flower bud differentiation of Camellia oleifera.In the period of flower bud physiological differentiation of Camellia oleifera,exogenous 6-BA can increase the content of ABA,ZR and GA₃in flower buds and reduce the content of IAA in flower buds.In the period of flower bud morphological differentiation,the exogenous 6-BA treatment make the content of ZR,GA₃,IAA in flower buds to reach the maximum in advance in petal formation.In the period of flower bud physiological differentiation,exogenous GA₃can improve the content of ABA,ZR,GA₃of flower bud;Spraying exogenous GA₃make the content of ZR,GA₃,IAA in flower buds to reach the maximum in advance in petal formation in flower bud morphological differentiation.（2）In the flowering period of Camellia oleifera,exogenous 6-BA decreased the content of ABA and GA₃in pistils first,then increased and then decreased.Stamen ABA and GA₃increased first and then decreased,and exogenous 6-BA makes the content of IAA in the hermaphrodite rise first and then decrease.Exogenous 6-BA increased the content of ZR in the pistil,and the content of ZR in the stamens increased first and then decreased.Exogenous GA₃increased the content of GA₃and ZR in pistil,and the ABA content of pistil increased first and then decreased,and the content of IAA in pistil decreased first and then increased.（3）Exogenous 6-BA and GA₃all reduced the content of soluble sugar in flower bud differentiation.In the experimental range,the effect of exogenous 6-BA treatment on the soluble protein content of Camellia oleifera leaves showed a certain trend of growth and decline,which was not significantly different from the CK.Exogenous GA₃increased the content of soluble protein in leaves during flower bud differentiation.（4）Exogenous 6-BA and GA₃can significantly increase the flower bud differentiation rate and fruit set rate of Camellia oleifera.In the experimental range,the effect of 500 mg/L exogenous 6-BA on the flower bud differentiation rate of Camellia oleifera was the best,and the flower bud differentiation rate increased with the increase of exogenous 6-BA concentration.In the future,further research is needed to solve how the flower tea bud differentiation rate changes when the spray concentration is greater than 500 mg/L.（5）During the physiological differentiation of Camellia oleifera,exogenous 6-BA was positively correlated with the flower bud differentiation rate.There was a significant positive correlation between exogenous 6-BA and the content of ABA in the physiological differentiation of flower buds.There was a significant positive correlation between ABA content and flower bud differentiation during flower bud physiological differentiation.During the physiological differentiation of Camellia oleifera,exogenous GA₃was negatively correlated with the flower bud differentiation rate of Camellia oleifera.Exogenous GA₃was significantly positively correlated with the content of ABA,GA₃and ZR during flower bud physiological differentiation.There was a significant positive correlation between the content of ABA and GA₃and the rate of flower bud differentiation during flower bud physiological differentiation.There was a significant positive correlation between the source ZR content and flower bud differentiation rate during flower bud physiological differentiation.（6）In the flowering period of Camellia oleifera,exogenous 6-BA was positively correlated with the fruiting rate.Exogenous 6-BA was significantly positively correlated with ZR in the initial wilting stage.There was a significant positive correlation between the ZR in the initial wilting stage and the fruit setting rate.Exogenous 6-BA was significantly positively correlated with the pistil IAA in the sorghum-containing period,and the pistil IAA was significantly positively correlated with the fruit setting rate.Exogenous 6-BA was significantly positively correlated with the pistil IAA in the sputum-containing period.The pistil IAA in the sputum-containing period was significantly positively correlated with fruit set rate.Exogenous6-BA was significantly positively correlated with stamens GA₃in the sorghum-containing period,and there was a significant positive correlation between stamen GA₃in the sorghum-containing period and fruit setting rate.Exogenous 6-BA was significantly positively correlated with stamen GA₃in the semi-open period,and there was a significant positive correlation between stamen GA₃in the semi-open period and the fruit setting rate of Camellia oleifera.Exogenous GA₃was negatively correlated with the fruit setting rate of Camellia oleifera.0-100 mg/L exogenous GA₃was positively correlated with the fruit seting rate of Camellia oleifera.The best exogenous GA₃concentration for improving the fruit set rate of Camellia oleifera remains to be further tested and studied.