Effect Of Hydrogen Rich Water On Wound Healing In Dogs

Skin trauma is a common surgical disease in the clinical practice of small animals.It is often accompanied by inflammation and oxidative stress in the healing process,sometimes it is easy to cause the phenomenon of wound not healing or delayed healing.The existing studies show that hydrogen molecules have the biological effects of anti-oxidation,anti-inflammatory and anti apoptosis.Therefore,the purpose of this study is to evaluate the effect of hydrogen rich water on skin wound healing in dogs.12 beagles were randomly divided into control group and experimental group(n=6).The experimental group regularly drank hydrogen rich water 2 times a day,and the control group drank distilled water.A full-thickness wound model of skin tissue was established on both sides of the midline of the dog's back.After modeling,observe the condition of the wound every day,record the bleeding,edema,exudation,scab and healing of the wound,and determine the final healing time of the wound.The wounds were photographed on day 0,3d,7d,14 d,21d and 28 d respectively.The images were analyzed by Image J software and the area of the wounds was measured to calculate the wound healing rate.The number of fibroblasts,inflammatory cells,neovascularization and epithelization thickness were observed in the histopathological section of the wound skin.The contents of MDA and SOD in the skin homogenate were measured.The contents of Nrf-2,HO-1,NQO1 and intravascular were measured The gene expression of VEGF and PDGF.The time of wound healing was 21 ± 0.75 d in the control group and 18 ± 0.62 d in the experimental group,which was less than that in the control group(P<0.05).At the same time,the average healing rate of the experimental group was significantly higher than that of the control group(P<0.01).According to the results of histopathological observation of wound skin,on the 7th day,the average number of neovascularization in the experimental group was significantly higher than that in the control group(P<0.01).On the 21 st day,the blood vessels in the experimental group basically disappeared,which was lower than that in the control group(P<0.05).On the 14 th day,the average thickness of epidermis in the experimental group was smaller than that in the control group(P<0.05),and on the 21 st day,the average thickness of epidermis in the experimental group was significantly smaller than that in the control group(P<0.01).The content of MDA and SOD in the skin homogenate of the wound was measured.The results showed that on the 7th day,the content of MDA in the control group was higher than that in the experimental group(P<0.05).On the 14 th day,the content of MDA in the control group was significantly higher than that in the experimental group(P<0.01).On the 7th,21 th and 28 th day,the content of SOD in skin tissue of the control group was lower than that of the experimental group(P<0.05).The results of real-time fluorescent quantitative PCR showed that the expression of nrf-2 gene in the experimental group was significantly higher than that in the control group on the 7th and 21 st day(P<0.01).On the 7th and 14 th day,the expression of HO-1 gene in the experimental group was significantly higher than that in the control group(P<0.01).On the 7th and 21 st day,the expression of NQO1 gene in the experimental group was significantly higher than that in the control group(P<0.01).On the 7th and 14 th day,the expression of VEGF and PDGF gene in the experimental group was significantly higher than that in the control group(P<0.01).The experimental results show that hydrogen rich water can accelerate wound healing by reducing inflammatory reaction,increasing the number of new blood vessels,accelerating wound epithelization,accelerating wound healing rate and shortening wound healing time.