| Qingpu granule is a modern Chinese medicine granule developed by chengdu qiankun animal pharmaceutical co.,LTD.This experiment mainly explored the effects of Qingpu Granules on some blood indexes and antipyretic effects of piglets.Experiment 1: Effect of Qingpu granules on blood routine and blood biochemical parameters of pigletsThirty 35-day-old Changbai piglets were randomly divided into 4 groups.In addition to the control group,the experimental group added 1、3、5 g/kg body weight of Qingpu granules to the piglet diet for 7 days.On the 0th,8th,and 13 th day,5 piglets were randomly selected from each group,and 5 mL/pig was collected from the anterior vena cava before morning feeding to detect blood routine and blood biochemical indicators.The results showed that there was no significant difference in blood routine and blood biomarkers between the pigs in the experimental group and the control group.Experiment 2: Establish LPS heat model to determine the optimal heating dose and body temperature changeSixteen 35-day-old Changbai piglets were randomly divided into 4 groups.In addition to the control group,the experimental group was injected intraperitoneally with 40,50,60μg/kg body weight LPS,and then injected once after 12 hours to continuously monitor and record changes in body temperature for 72 h.The results showed that after 2 hours of LPS injection,the body temperature of all piglets began to increase gradually.The body temperature of the piglets in the low and middle dose groups increased to the highest temperature 6 hours after the first injection of LPS,and the body temperature gradually increased after the injection of LPS at 12 hours.In the fluctuating state,the body temperature returned to normal in the low-dose group from 42 h,and the body temperature in the middle-dose group remained above 39.63℃In the high-dose group,the body temperature of the piglets changed the most.After the first injection of LPS,the body temperature remained above 40.06 ℃,and reached the peak at 8,20,and 60 h,respectively.Considering the medium dose LPS(50μg/kg body weight),the heat effect of the white piglets was the best.The time of Filling Chinese medicine for the first time was 6hours after the first injection of LPS.Experiment 3: Antipyretic effect of Qingpu granules on LPS-heated pigletsSixty 35-day-old Changbai piglets were randomly divided into normal control group,positive control group,Gaoqin oral solution group and Qingpu granules low,medium and high dose groups.Except for the normal control group,LPS was inoculated(the optimal pyrolysis dose determined by test 2 was 50 μg/kg body weight),and 4 drug groups were given LPS for 6 hours after inoculation of the piglets,respectively.Qingpu granules 0.5,1.0,1.5g/kg body weight,continuous administration for 3days,daily observation of clinical symptoms and body temperature;Calculate average daily feed intake,daily gain,and feed-to-weight ratio;On the 0th,2nd,4th and 6th day,5 piglets were randomly selected from the anterior vena cava for blood collection of 3 mL/pig.After separation of serum,TNF-α,PGE2,MIP-1,IL-1β,IL-6 and IL-8 were detected;On the 4th day,2 piglets were randomly selected from Positive control group and Qingpu Granule Low Dose Group,paraffin sections were made from lung,liver,kidney and spleen to observe the pathological changes of the corresponding tissues.The results showed that: 1)LPS can maintain the average body temperature of piglets within the range of 39.90~41.87℃within 96 h.After LPS,the body temperature of the piglets in the high-dose group and the Gaoqin oral solution group,the Qingpu granules and the low-dose group of Qingpu granules were respectively drops below 39.5℃ after 36,48,60h;2)The average daily feed intake and average daily gain of piglets in each group were lower than those in the normal group,which was higher than that in the positive control group.The middle and high dose groups of Qingpu granules were significantly higher than the Gaoqin oral solution group and the Qingpu granules low dose group.3)LPS can significantly increase the levels of TNF-α,PGE2,MIP-1,IL-1β,IL-6 and IL-8 in the serum of piglets.On the 2nd day,the above-mentioned cells in the low,middle and high groups of Qingpu granules and the Gaoqin oral solution group.The factor content was significantly lower than that of thepositive control group,and the dosage of each of the Qingpu granules showed dose dependence.Except for TNF-α in the middle dose group of Qingpu granules,the content of the above six cytokines in the middle and high dose groups of Qingpu granules was significantly lower than that of the Gaoqin oral solution group and the Qingpu granules low dose group;The lower group MIP-1 and IL-8 of Qingpu granules were also lower than the Gaoqin oral solution group from the 2nd day.The IL-6 in the middle dose group of Qingpu granules started from the 4th day and the MIP-1 in the high dose group started from the 2nd day.The IL-6 began from the 4th day,and the IL-1β and IL-8 were lower than the normal control group at the 6th day.Experiment 4: Observation on the effect of Qingpu granules in the treatment of Haemophilus parasuis.90 pigs diagnosed as Haemophilus parasuis by bacterial isolation and PCR were randomly divided into 3 groups(flufenicol group,Qingpu granules combined group,Gaoqin oral solution combined group).Piglets in each group were intramuscular injection of florfenicol 20mg/kg body weight,the two combined groups were respectively administered 1mL/kg body weight Gaoqin oral solution,1.0g/kg body weight Qingpu granules,2 times a day for 3 consecutive days.7 days of trial,statistical efficacy.The results showed that the efficacy of Qingpu granules combined with florfenicol in the treatment of Haemophilus parasuis was better than that of florfenicol and Gaoqin oral solution combined with florfenicol.Conclusions:(1)Qingpu granules can be safely given to piglets by feeding twice a day at less than 5g/kg body weight;(2)The dosage of Qingpu granules and Gaoqin oral solution can effectively inhibit PGE2,TNF-α,MIP-1,IL-1β,IL-6 and IL-8 in LPS(50μg/kg body weight)pyrogenic piglets.The Qingpu granules group had a dose-dependent effect,and the middle and high dose groups were superior to the Gaoqin oral solution group;(3)1.0g/kg body weight Qingpu granules combined with florfenicol treatment of Haemophilus parasuis,the effect is better than florfenicol alone.The results of this test indicate that Qingpu granules can be safely used in production practice,and the recommended dose is1.0g/kg body weight. |
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