| Tumor diseases and cardiovascular diseases are the two major threats to health and life,but the cross-cooperation between these two disease research fields has not caused clinical attention.Breast cancer is the most common cancer affecting women worldwide,and most women are treated with anti-cancer drugs.Unfortunately,cancer treatments cause cardiotoxicity in general and can be life-threatening in some cases.At present,cardiotoxicity has become the biggest resistance and hidden danger in the treatment of cancer.Therefore,this experiment explored the influence and mechanism of artemisinin in the pathogenesis of myocardial ischemia in breast tumors.The model of myocardial ischemia in nude mice transplanted with canine mice was established to study the combination of artemisinin on these two diseases.The impact and mechanism of time.The canine mammary gland tumor cell lines in the logarithmic growth phase were obtained in the same growth state,and 20 nude mice were sequentially injected with 0.2 ml of a cell suspension having a concentration of 1 × 10 7 cells/ml.The nude mouse model was randomly divided into 5 groups,4 in each group.Artemisinin was administered by intragastric administration from the 2nd day after inoculation of the tumor.The concentration was high dose group: 200 mg/kg,medium dose group: 100 mg/kg,low dose.Group: 50 mg/kg,the dose was 0.1 ml,and another set of blank control group.Model group:0.1 ml of sodium carboxymethylcellulose(CMC)+ normal saline was administered for 21 days.The body weight of nude mice was recorded every 3 days,and tumors were formed after 7 days,and the tumor volume was measured every 2 days.From the 15 th day onwards,each mouse was injected subcutaneously with isoproterenol 5 mg/kg for myocardial ischemia.During the period,the mice developed open mouth breathing,decreased mobility,mental depression and other myocardial ischemia.The content of CK and LDH-L showed that the blank group had higher content than other components,indicating that the rabbit model of myocardial ischemia was successfully established in nude mice.The nude mice were sacrificed on the 22 nd day(24 hours after the last administration),the transplanted tumor tissues were exfoliated,and the heart was collected.The tumor suppression rate was calculated by weighing the tumor;the heart weight was weighed,the heart-to-body weight ratio was calculated;the tumor volume change curve after the long tumor was drawn;the transplanted tumor tissue and pathological changes were observed by HE staining;the creatine kinase in the serum was detected by the rate method.The content of(CK)and lactate dehydrogenase(LDH-L);the detection of the expression of Sirt3/Src/Fak pathway-related factors in transplanted tumor tissue and the expression of Sirt3/Foxo3pathway-related factors in cardiac tissue by real-time PCR Variety.1.On the 7th day,the canine mammary gland tumor cells inoculated on the right suboccipital region of nude mice grew macroscopic tumors with a tumor size of about 2 mm× 2 mm,indicating that the tumor model was established successfully and the tumor formation rate reached 100%.The results of tumor volume and tumor weight were as follows: model group>low dose group>middle dose group>high dose group.The inhibition rate of tumors in low,medium and high dose groups was 21.1%,47.9% and 56.1%,respectively.3.HE staining of transplanted tumors showed that compared with the model group,the artemisinin treatment group had a nuclear fission phase,a nuclear atypia,a small cancer nest,and an increase in inflammatory cells,and the middle dose and high dose groups were more obvious.HE staining of cardiac tissue showed that myocardial fibrinolysis,myocardial nuclear lysis,deep cytoplasmic staining,myocardial hemorrhage,and endocardial cell shedding were reduced in the artemisinin-treated group,and ventricular inflammatory cell infiltration near the endocardium was compared with the model group.Reduced,vacuolization is reduced.4.The results of serological tests showed that the expression levels of CK and LDH-L in nude mice serum were in the model group > low dose group > middle dose group > high dose group > blank group.5.The results of real-time PCR showed that for tumor tissues,the expression levels of Fak,Src and Sirt3 related to tumor tissue growth were affected,and the model group>low dose group>middle dose group>high dose group.The expression levels of Foxo3 and Sirt3 were related to cardiac tissue,and the model group>low dose group>middle dose group>high dose group>blank group.Conclusion: Artemisinin can significantly inhibit the anti-tumor effect and improve myocardial ischemia in the transplantation of canine breast tumor cells in nude mice.It may protect the heart by regulating Sirt3 and Foxo3 gene expression,and regulate Sirt3.,Src,Fak gene expression inhibits tumors. |
