| Thuja sutchuenensis Franch.is an evergreen tree of the genus Cypress.It was rated as a wild extinct species and an extremely endangered species by IUCN in 1977and 2003.In 2015,during the second survey of wild plant resources in the country,the wild Thuja sutchuenensis Franch.population was first discovered in Sichuan.As a living fossil tree,the cypress is excellent in wood and has important scientific research and application value.However,the wild Thuja sutchuenensis Franch.has a very low seed setting rate and a high rate of abortion,so the rescue work for the cliffs in Sichuan is imminent.Due to the lack of saplings,the selection of materials is limited.This experiment is based on the adult wild cypress in XuanHan County.On the basis of field investigation,through the cutting method,tissue culture and other test methods to explore the feasible technical means of artificial breeding of Thuja sutchuenensis Franch.,provide theoretical basis and related technical methods for the expansion and commercial production of Thuja sutchuenensis Franch.in Sichuan.The survey and research results are as follows:?1?The main plant in the wild community of Thuja sutchuenensis Franch.are:consists of fern are fern families?Pteridiaceae?and Gleicheniaceae?Gleicheniaceae?,2 species,2 genera and 2 Families;Gymnosperms found a total of Cupressaceae Cupressaceae,taxus chinensis?Taxaceae?and Pinaceae?Pinaceae?3 4 genera,5 kinds of;In addition,33 families,59 genera and 69 species of angiosperms.A total of 76species of 35 families and 65 genera.Can be divided into five groups,respectively,Pinus massoniana Lamb.-Fargesia spathacea Franch-Cyperus rotundus L.community,Pinus massoniana Lamb.-Fargesia spathacea Franch-Pteridophyta community,Quercus spinosa-Fargesia spathacea Franch-Cyperus rotundus L.community,Thuja sutchuenensis Franch.-Fargesia spathacea Franch-Cyperus rotundus L.community,Thuja sutchuenensis Franch.-Fargesia spathacea Franch-Pteridophyta community.?2?After weighing and calculating,sumac XuanHan county township of wild Thuja sutchuenensis Franch.seed kernels per 0.6508 g?collection in XuanHan county,February 16th?.Other documented Thuja sutchuenensis Franch.grain,in turn,is0.9056g?collection in Kai county,August 28?,0.7967g?collection in Kai county,September 20?,1.4962g?collection in Kai county,25 October?.?3?All known wild Thuja sutchuenensis Franch.xuanhan county sample area,in the back of soil samples were measured in a large number of elements in soil potassium,calcium,and trace elements manganese,sodium,iron,zinc,the content of total nitrogen and total phosphorus,compared to the number of inner wild Thuja sutchuenensis Franch.,correlation analysis and found K?Ca?Fe?Mn?Zn,the content of total phosphorus and total phosphorus and Thuja sutchuenensis Franch.in sample area there was no significant correlation,the content of potassium and Thuja sutchuenensis Franch.negatively correlated with the number of relationshIPs within the sample area.?4?In chengdu university of technology for outdoor cutting breeding experiments,the preliminary conclusion:spring march is most suitable for cutting rooting rate of41.67%,the summer cutting rates are highest in June is the fastest time of death effect is very poor;The best effect in the matrix combination is peat soil:vermiculite:perlite=1:1:1?V,V,V?;Water treatment and low concentration indolebutyric acid root has the obvious effect of Thuja sutchuenensis Franch.,sort of different hormone treatment combination is Water Treatment>IBA 2000 mg·L-1>IBA 1000 mg·L-1>IBA 3000mg,L-1.In addition test showed that callus and take root and no significant correlation between.?5?Cupressaceae plants surface grease is difficult to remove,laboratory pollution rate was 100%,the magnetic stirrer can be used to improve this situation,a month after the pollution rate was 75%.Species found in the test medium has a significant effect on shoot proliferation,and different kinds of hormone treatment only for 1/2 SH bud proliferation have a significant impact on culture medium,add 2 mg·L-1 2-IP or 2mg·L-1 ZT growth regulator of 1/2 SH for the most suitable for shoot proliferation medium;Shoot elongation growth the most suitable culture medium for DCR.?6?Thuja sutchuenensis Franch.have excellent ornamental features,its rich landscape application form,can be lonely,to plant,clump planting,group planting configuration,integrating view form,foliage,also can match with other garden plants,buildings or other structures essay use,have a certain application prospect. |
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