| Paa boulengeri is a kind of large edible frog of mountain district with delicious taste, rich nutrition,tonic effect and medicinal value, and has a better development prospect. Biological characteristics and ecological culture techniques of cultured Paa boulengeri were systematically studied by using groundwater in the low-altitude area from April 2008 to March 2010, which were on the basis of traditional culture of frogs and started from ecology and systems engineering. The main conclusions are as follows:1. Paa boulengeri adult frogs are 102.4±12.7mm in length,177.5±32.4g in weight. Adult frogs prey at night, the peak of which is between 6 p.m. and 8 p.m. It is one-time satiation and of digestion and absorption during the day instead of eating. The spawning is usually at 1 a.m. to 9 a.m., which is very long and discontiguous, while the breeding persist for several hours or even 1～2d.2. Under the condition of artificial cultivation female Paa boulengeri lay 218±67 eggs each time, the diameter of eggs is 3.98±0.12mm. Under the condition of artificial cultivation, it is the spawning peak from mid-May to the end of June, which accounts for more than 60% in annual fecundity. Under the artificial feeding and management, the fertilization rate of natural reproduction eggs is relatively high, more than 93% on average, while hatching rate reaches 80%. Incubation time decreased gradually with the temperature increases while malformation rate increases.3. Tadpoles are 10.98±1.21mm at hatching. The young frogs which just complete metamorphosis are 1.98±0.12cm in length,1.24±0.10g in weight, Under the artificial cultivation in high density tadpoles have the habit of cannibalism, The individuals after metamorphosis are smaller than the ones in the wild, while the abnormal time is in advance. Young frogs are always gregarious, first feeding of which is normally 7～10d after the completion of the metamorphosis, and 7 p.m～8 p.m at night is the peak of their feeding. The metamorphosis rate of tadpoles reached 88% in this test. In addition, the multi-vegetable diet is fed to delay metamorphosis, and animal diet is fed to speed up the process of metamorphosis.4. Four kinds of initial feeding of young Paa boulengeri were contrasted, and it showed that Chironomidae midges larvae was the best initial feeding. The hibernation experiment for young Paa boulengeri in different size showed that the smaller was the individual, the higher the overwintering mortality rates. The weight loss of young frogs was about 6% after overwintering. The young frogs increased from 8.31±1.02g to 35.21±3.62g in weight in overwintering test by controlling the temperature, while survival rate was 87%.5. Under the condition of artificial cultivation it will take Paa boulengeri about 1.5 years from 20-30g to more than 200g, and the growth pattern is a "slow-fast-slow" process. In the process of feeding adult frogs, it is the peak period of death for adult frogs in June,July and August, the number of deaths accounted for 43% of total, and the mortality rate of adult frogs in hibernation was 8%.In addition, this paper explored a rather perfect Ecological Culture Techniques combined pond breeding with shed breeding. In allusion to the current culture status of Paa boulengeri combined with experience in frog-breeding, author raised the operational procedures on ecological culture of Paa boulengeri, and provided a reference for the promotion of breeding Paa boulengeri in different areas.
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