Chemical Constituents On The Trunks And Roots Of Thuja Sutchuenensis

Plants of the genus Thuja were traditionally used to treat a variety of diseases.For resisting the force of the poor environment to survive,a series of structural unique secondary metabolites may be developed in T.sutchuenensis Franch..In order to clarify the chemical constituents and bioactivities of T.sutchuenensis,the trunks and roots were selected to be conducted the following phytochemical study.With this effort,five new compounds were successfully isolated and evaluated for their antimicrobial and in vitro cytotoxic activities against a panel of bacteria and four of human tumor cell linesDetailed phytochemical investigation on the trunks and roots of T.sutchuenensis had led to the isolation of 29 natural products by various chromatographic methods.Their structures were fully characterized and determined on the basis of spectroscopic analyses(HRESIMS,1HNMR,13C NMR,COSY,HMQC,HMBC,NOESY),and their structures was shown to belong to a variety of structural classes.All of the 29 natural products could be divided to seven stilbenes,thujasutchins A-B(T1-T2),ugonstilbene B(T3),4-(3-methylbut-2-en-l-yl)-5-phenethylbenzene-1,3-diol(T4),3-methoxy-4-(3-methylbut-2-en-1-yl)-5-phenethylphenol(T5),longistyline A(T6),and cajanolactone A(T7);five phenolic compounds,thujasutchins C-D(T8-T9),frambinone(T10),4-hydroxybenzaldehyde(T11),and 3-(8Z-tridecenyl)-phenol(T12);three sesquiterpenes compounds including thujasutchin E(T13),mayurone(T14),and 1,7-diepi-a-cedrenol(T15);eight flavones including pinocembrin(T16),pinostrobin(T17),myricetin(T18),(2R,3R)-(+)-4-0-methyldihydroquercetin(T19),kaempferol(T20),5,7,3,′4′-tetrahydroxy-3-methoxy-flavone(T21),quercetin(T22),and dihydrokaempferol(T23);six lignans,7-oxohinokinin(T24),(-)-hinokinin(T25),heliobuphthalmin(T26),(-)-hibalactone(T27),(7E)-7,8-dehydroheliobuphthalmin(T28),and(-)-hinokiresinol(T29).Among them,T1-T2,T8-T9,and T13 were new compounds Moreover,all the compounds except compounds T20 and T22 were obtained from T.sutchuenensis for the first timeAll the new compounds were evaluated for antibacterial activities against Staphyloccocus aureus,Escherichia coli,and Methicillin-resistant S.aureus.Compounds T1,T8-T9,and T13 were evaluated for in vitro cytotoxic activity against SF-268,MCF-7,HepG-2,and A549 tumor cell lines.Unfortunately,all of them failed to show significant antibacterial activities at 500μg/mL and noticeable cytotoxicity at a concentration of 100 μM.