The Study On Effect Of Flavonoids From Allium Mongilicum Regel Blood Biochemical Parameters And Intestinal Mucosal Immune In Meat Sheep

In this paper,the effects of flavonoids from Allium Mongilicum regel on the growth performance,blood-related biochemical indexes and intestinal immunity were studied.75 small tail Han ram sheep of 6 months old and similar weight were randomly divided into 5 groups(n=15),fed with basal diet(control group),basal diet+Allium Mongilicum regel powder 20 g/d.(Flavonoid low-concentration group),basal diet+22 mg/kg Allium Mongilicum regel flavonoids(flavonoid middle-concentration group),basal diet+33 mg/kg Allium Mongilicum regel flavonoids(flavonoids high-Concentration group).Pre-feeding period is 15 d,and feeding period is 60d.The feed intake of each group of sheep was recorded every day after the start of the trial period.The small tail Han ram sheep were weighed at the 0 d,15 d,30 d,45 d and 60 d of the experiment.Four sheep were collected in each group to test the total protein(TP),albumin(AIB)and globulin(GLB)in serum.At the end of the trial,three sheep were randomly selected from each test group,and sacrified to collect the duodenum,jejunum and ileum for observing the changes of intestinal villi height,crypt depth and V/C ratio.Then using the intestinal,jejunum,ileum intestine mucosa,we determinate the concentration of secretory immunoglobulin(SIgA).Using the contents of the cecum and colon,the number of E.coli and lactobacilli were determined.The test results are as follows:(1)The dietary intake,average daily gain and feed reward(P<0.05)were significantly increased when 20 g/d of perilla frit and 11 mg to 33 mg/kg of Allium Mongilicum regel flavonoids were added to the diet.And the feeding effect was the best after feeding for 45 days.(2)There was no significant difference in serum TP and ALB levels between Od,15d and 30d(P>0.05),and significantly increased at 45d and 60d compared with the control group(P<0.05).There was no significant difference in serum GLB level between 0,15,30 and 60 days(P>0.05),and significantly increased(P<0.05)at 45 days compared with the control group(P>0.05).(3)The content of SIgA in the duodenum and ileum mucosa was significantly lower than that in the control group(P<0.05).(4)Compared with the control group,the jejunum and ileal villus were significantly increased(P<0.05),but there was no significant difference in the height of jejunum villus between the test groups(P>0.05).There was no significant difference between the duodenal segment and the low concentration of flavonoids(P>0.05),and the other three test groups were significantly improved(P<0.05).The depth of the duodenum,jejunum and ileum were significantly decreased(P<0.05),but the ratio of V/C was significantly increased(P<0.05),there was no significant difference between the Allium Mongilicum regel group and the flavonoid high-concentration group(P>0.05).(5)Compared with the control group,the number of cecal and coliform Escherichia coli in the test group was significantly decreased(P<0.05),while the number of lactobacilli was significantly increased(P<0.05).