DNA Methylation And Alternative Splicing Affect FBXW11 Gene Expression And Sperm Motility In Holstein Bull Testis

Molecular breeding is a key means for improving dairy cattle herd.For the cow population,since the genetic contribution of the bulls to the dairy population exceeds 70%;the effect of semen quality traits on the dairy population is self-evident.In the early stage of this study,high throughput sequencing technique was used to sequence semen samples with different quality,and the genes expressed differently in the samples with different semen quality were selected as candidate genes(P<0.05).In order to screen the candidate genes which affect the semen quality of bulls as molecular genetic markers,the molecular biological techniques were used for later verification.Our study showed differential expression of FBXW11 in different Chinese Holstein cows(P<0.05),and three splice isoforms were found in the study.Alternative splicing is important for regulating gene expression and enriching the number of transcripts(P<0.05),and the absence of the full-length FBXW11 gene is detected in the sample,which indirectly demonstrates the effect of alternative splicing on FBXW11 and thus on semen quality.Immunohistochemistry and protein immunoassay showed that the expression of FBXW11 protein in different semen quality samples was different(P<0.05),while the fluorescence quantitative test and semi-quantitative test analysis showed that the expression of FBXW11 gene was different(P<0.05),and analysis.The mechanism by which alternative splicing affects the quality of bull semen and the causes of alternative splicing.