| Hepatocyte toxins such as aflatoxin,carbon tetrachloride,and sulfonamide can often cause acute toxic hepatitis in dogs.Although the clinical incidence is not high,the lack of special antidote is serious.The canine acute liver injury model is a commonly used model for studying the efficacy test of liver-protecting drugs.Based on the establishment of a model of acute liver injury in dogs,this study preliminarily explored the protective effect of traditional Chinese medicine decoction and hepatic protectant A oral solution on acute liver injury in dogs.In the first experiment,18 healthy Chinese rural dogs were randomly divided into 3 groups,6 in each group,and 50%CCl4 peanut oil solution was injected into the abdominal cavity?group I?and subcutaneous multiple points?group II?at 1mL/kg.Normal control in the control group.Clinical examination,blood routine,serum biochemical indicators,abdominal X-ray,ultrasonography and ultrasound-guided liver biopsy were performed.The results of clinical examination showed that the dogs in group I and group II showed clinical symptoms such as depression,decreased appetite,and yellowing of urine color,and there were 2subcutaneous cysts in group II.The results of blood routine showed that WBC in group I and II increased,and the difference was significant?P<0.05?,and RBC and PLT were slightly decreased.The results of serum biochemical indicators showed that serum ALT,AST and ALP were significantly increased in group I and group II compared with the control group?P<0.05?.Abdominal X-ray films showed that there was no significant difference in liver volume between dogs in groups I and II?P>0.05?.Ultrasound sonogram showed that on the 2nd day of the experiment,both groups I and II showed a slightly rounded liver edge and a decrease in liver parenchyma echo.On the 7th day,the liver parenchyma was not smooth and the scattered echo was enhanced in group I and group II.The histopathological examination showed that the hepatocytes in group I and II were swollen on the 2nd day,but the degree of hepatocyte degeneration in group II was smaller than that in group I.On the 7th day,hepatocytes in group I were necrotic,and there were red blood cells in connective tissue interstitial infiltration.Group II hepatocyte necrosis,but less than group I.In the second experiment,30 healthy Chinese rural dogs were randomly divided into 5 groups:high-,medium-,and low-dose groups of liver-protecting drugs,positive control group and negative control group.In addition to the negative control group,the other group used the method of intraperitoneal injection to establish a model of acute liver injury in dogs.The dogs in the high,medium and low dose groups were treated with 1 mL/kg,0.5 mL/kg and 0.25 mL/kg.hepatic protectant A,2 times a day?interval 12 h?.Serum biochemical indicators and ultrasound-guided liver biopsy were used for drug efficacy evaluation.The results showed that serum ALT in the high and middle dose groups was significantly lower than the positive control group?P<0.05?.Histopathological examination showed that the hepatocytes in the high,middle and low dose groups and the positive control group had different degrees of edema on the 2nd day of the experiment.On the 7th day of the experiment,the hepatocytes in the high,middle and low dose groups and the positive control group were different degree of necrosis.In summary,this test can successfully establish a model of acute liver injury by multiple examinations of subcutaneous injection of CCl4 and single intraperitoneal injection of CCl4.Compared with intraperitoneal injection,the method of subcutaneous injection has large dispersion of serum biochemical data,poor stability,and may form subcutaneous cysts.Serum biochemical markers and ultrasound-guided liver biopsy are more sensitive to acute liver injury in dogs.In this experiment,0.5 mL/kg of hepatic protectant A has a certain therapeutic effect on acute liver injury induced by intraperitoneal injection of 50%CCl4solution. |
PDF Full Text Request