Testicular Developme Nt Of Ningdu Yellow Chicken

Ningdu Yellow Chicken is a high quality local chicken in China.It has the characteristics of delicious meat,early sexual maturity,genetic stability,temperament,clustering and strong resistance,but its reproductive performance is low.In recent ten years,the researches on the reproductive performance of N ingdu Yellow C hicken were mainly focused on the hen,and most of the research on the performance ofN ingdu Yellow Chicken were studied by semen quality,but the semen quality was appearance,We knew little about the mechanism oftesticular development process.The 547 N ingdu Yellow C hicken at 50 w of age were usedin this study.Weekly we measured weight,testicular weight,tibial length,tibia,crown height and more.Obvious,we found different size of thetesticular.Then we do testicular paraffin sections.The results showed that the changes of the internal structure of the testes.After that,we measured the blood levels of FSH,LH and T by Radioimmunoassay(RIA).At last,we used q-PCR to measured FSHR,LHR and AR expression level.Through the above experiment found:(1)N ingdu yellow cock testicular development of the critical period: early 0 to 3 weeks of age for the solid lumen,3 weeks of age began to appear lumen.After 5 weeks of age,the diameter of the fine tube was significantly increased,the lumen was generally formed,the lumen was smaller,the gap of seminiferous tubules was reduced,the density of testicular interstitial cells began to decrease,and the stromal cell volume increased.At the 8th week of the second development stage,the development of the seminiferous tubules was not obvious at 8 weeks of age,and the testicular weight was not obvious.The main spermatogonia was the development of spermatogonia,Began to significantly gain weight.15 weeks of age began to produce sperm and the test has been developed but the testicular weight is still increasing,26 weeks of sperm count began to increase,36 weeks of age to reach the maximum testicular weight 40.08 g,37 to 44 weeks The pulp tube is filled with sperm.Combined with hormone concentration speculation 29 to 46 weeks to produce sperm peak.43 weeks after the testicular weight began to decline,but the number of sperm has not yet reduced.46 weeks of fine seminiferous tubules within the sperm count was significantly reduced,testicular began to aging.50 weeks of age lumen almost healed,but the cells at all levels are still intact.(2)testes were generally greater than the right testes on the left side of the testis,while the right testis FSH receptor,LH receptor and testosterone receptor transcripts were generally higher than the left testis.In the early left testicular development slightly faster than the right testis,the development of the left or right testicular tissue structure,spermatogenic function is no significant difference.(3)Pearson correlation analysis showed that the total weight of testis was significantly correlated with body weight,tibial length,tibia,crown height,crown length,crown thickness,meat length,flesh thickness,FSH,LH and T,Long,tibia,crown height,crown length,crown thickness,meat length,meat thickness and T were positively correlated with FSH,LH was negatively correlated.And the optimal model equation of testis weight was obtained by SPSS optimal model analysis.