Effects Of Ambient Temperature On Noxious Gases Emission From Chamber And Their Impact On Plasma Biochemical Parameters Of Sheep

Temperature is one of the most important factors of environmental quality assessment.The purpose of this study was to investigate effects of temperature on oxygen consumption?O₂?,carbon dioxide production?CO₂?,methane emission?CH₄?,ammonia emission?NH₃?,hydrogen sulfide concentration?H₂S?of metabolism chamber,and environmental microbial community,plasma biochemical parameters and some harmful gas emission from faeces as well.The results showed as follow.Experiment 1:Effects of temperature on harmful gases concentration and environmental microbial community of metabolism chamber and plasma biochemical parameters of sheep:A single factor design was used with three environmental temperature range of 10¹5?,20²5?,and 30³5?.Twenty-four healthy crossbreed rams?Dorper?×Small tail Han-Yang??with similar weight?35.34±1.07 kg?were randomly divided into 3 groups,8 replicated each group.The trial lasted 17 days,including 7-d acclimation and 10-d experimentation.Rams fed at single breath metabolism chamber.During the trial period,the atmospheric and indoor O₂,CO₂ and CH₄ concentrations were automatically recorded by the probe each 3minute.NH₃ and H₂S concentration was measured by samples pumped from metabolism chamber three times?11:00,14:00 and 17:00?each day.Count of E.coli,salmonella,staphylococcus aureus and total bacteria in metabolism chamber was measured at 7:00 and 18:00 daily.At the morning of 10-d,5 mL vein blood was collected from rams to determine the cortisone?CORT?,concentration of immunoglobulin?IgG?and creatine kinase?CK?and lactate dehydrogenase?LDH?activity.The results showed that the emission of CH₄ and NH₃ in metabolism chamber was significantly increased with the increase of temperature?P<0.01?,but there was no significant effect on CO₂ and H₂S emissions?P>0.05?.Count of E.coli at 30³5?condition was significantly higher than others?P<0.05?.Count of salmonella and total bacteria at 30³5?and 20²5?condition was significantly higher than that of 10¹5?group?P<0.05?.The temperature had no significant effect on the adrenal corticosterone?CORT?,immunoglobulin?IgG?,creatine kinase?CK?and lactate dehydrogenase?LDH??P>0.05?.Experiment 2:Effects of temperature on harmful gases emissions from faeces:A single factor design was chosen with three temperature range,10¹5?,20²5?,and 30³5?,three repetition each group.From 4d to 7-d of experiment 1,all over of faeces were collected and mixed.Then 5 kg faeces sample was chosen as each repetition,and put them into mini metabolism chamber to measure harmful gases emission from faeces at different temperature.The trial lasted 5d.The results showed that O₂ consumption and CO₂ emission rose firstly and decreased soon with increase of temperature,with a peak value of 15821 mg/m³ and 17262 mg/m³ at 48 h.NH₃emission at 30³5? condition was significantly higher than 10¹5? and 20²5? group by 44.03%and 40.51%?P<0.01?,and H₂S emission significantly higher than that of groups by 59.84%and 34.64%,respectively?P<0.01?.