Molecular Epidemiological Suevey Of Bovine Theileriosis In Yanbian, Jilin

Bovine theileriosis is generally spread by ticks that inhabits bovine lymphocytes and erythrocyte.It causes fever,anemia,jaundice,and swelling of surface lymph nodes.In China,there are mainly three kinds of Theileria orientalis,Theileria sinensis and Theileria annulata.Bovine theileriosis is closely related to the active area and epidemic situation of ticks.The disease appears obvious regional and seasonal prevalence,and it is difficult to completely eradicate.Even severe infection can cause death of infected cattle and bring huge economic losses and potential threats to the cattle industry.To know the situation of theileriosis infection in Yanbian,Jilin Province,we designed specific primers based on the 18S rRNA gene of Theileria spp,the Tams-1 gene of T.annulata,the MPSP gene of T.orientalis,and the MPSP gene sequence of T.sinensis in GenBank.the molecular detection of bovine theileriosis was performed on 256 bovine blood samples collected from Helong,Hunchun,Longjing and Yanji in Yanbian,Jilin Province by PCR.The amplified genes were analyzed by sequence evolution analysis and insect species identification.The results showed that 256 bovine blood samples collected from four counties and cities in Yanbian,Jilin Province were detected by PCR for infection with bovine theileriosis;After sequence analysis of the amplified gene,it was determined that the infected parasite were T.orientalis and T.sinensis,and there was no T.annulata infection;the infection rate of T.sinensis was 37.5%(96/256),the infection rate of T.orientalis was 39.1%(100/256),mixed infection rate of T.orientalis and T.sinensis was 34.8%(89/256).This experiment provided scientific epidemiological data for the effective prevention and control of bovine theileriosis in Yanbian,Jilin Province.