Effects Of Chinese Herbal Medicine And Fructoolig Osaccharides On Growth And Immunity Of Japanese Seabass,Lateolabrax Japonicus

Feeding trials were conducted to investigate the effects of a Chinese herbal medicine(CHM)and Fructooligosaccharides(FOS)supplementation on the growth performance,digestive enzyme activity and non-specific immunity of Lateolabrax japonicas.As one of commercially important marine fish in China,Lateolabrax japonicas has been widely cultured in recent years.The main contents and results of the study are presented as follows:(1)The purpose of the first part was to study the effects of CHM supplementation on the growth performance,digestive enzyme activity and non-specific immunity of lateolabrax japonicas(initial weight 5.01±0.32 g).The fish were fed one of the six experimental diets containing [0 mg/kg(basal diet),4 g/kg(0.4%group),8 g/kg(0.8%group),16 g/kg(1.6%group),20 g/kg(2% group)] twice daily for 28 d.The fish were randomly divided into eighteen 120 L(6 groups with 3 replicate of 30 fish each)tanks equipped with close-recirculating culture systems.The results showed that the body crude protein(CP)content was the lowest in fish fed the basal diet,and 1.6% group was significantly higher than control group(P<0.05).There was no significant difference in survival rate(SR)among all of the treatment groups(P>0.05).Weight gain rate(WGR)and specific growth rate(SGR)of the 0.8% group were significantly higher than control group(P<0.05),while feed conversion ratio(FCR)and hepatopancreasomatic index(HI)of the 0.8% group were significantly loser than the control(P<0.05);The total protein(TP)content,protease,amylase(AMS)and lipase activity(LPS)in stomach of fish in 0.8% group were significantly higher than those of the control(P<0.05),and the protease,AMS,LPS in intestine of fish in 1.2% group were significantly higher than the control(P<0.05);The TP,lysozyme(LSM)and the alkaline phosphatase(AKP),acid phosphatase(ACP),total antioxidative capacity(T-AOC)activity in serum of fish in 1.2% group were all significantly higher than those of the control(P<0.05).The total superoxide dismutase(T-SOD),T-AOC,CAT(Catalase),AKP and ACP activity in liver of fish in 1.2% group were all significantly higher than those of the control(P<0.05),while GPT(Glutamic-pyruvic transaminase)in liver of fish in 1.2% was significantly lower than that of the control(P<0.05).Above all,the CHM is appropriate to add at the level of 0.8~1.2%,and accoding to the best fitting curve we can concludede the best level of CHM added into diets is 1%.(2)The purpose of the two part was to study the effects of FOS supplementation on the growth performance,digestive enzyme activity and non-specific immunity of lateolabrax japonicas(Initial weight 38.3±0.5 g).The fish were fed one of the six experimental diets containing 0 g/kg(basal diet),0.5 g/kg(0.05%),1 g/kg(0.1%),2 g/kg(0.2%),4 g/kg(0.4%),6 g/kg(0.6%)for 28 d.The fish were randomly divided into eighteen 120 L(6 groups with 3 replicate of 30 fishes each)tanks equipped with close-recirculating culture systems.The results showed that the body moisture and EE content were all lower than the control group and 0.6% group were significantly higher than control group(P<0.05).WGR and SGR of 0.1% group were significantly higher than the control(P<0.05)and FCR of 0.1% group were significantly lower than the control(P<0.05).The TP,LPS and protease activity in intestine of fish in 0.2% group were significantly higher than those of the control(P<0.05),while no significant difference was observed of the AMS(P>0.05).The LZM contents and AKP activity in serum in 0.2% group were significantly higher than that of the control(P<0.05),while TP and T-SOD has no significant differences among all of the experimental groups(P>0.05).In conclusion,we considered the FOS appropriate to add at the level of 0.1%~0.2%.