Establishment Of Regeneration Systems Of Several Meaty Plants

Plant market is very popular in recent years,more than meat,it looks small and exquisite,can purify air,and highly ornamental value.But because of the limitations of its own growth characteristics,some precious meaty plant is difficult in rapid propagation under natural conditions,some even seeds not knot,lead to more meat plant under natural conditions are gradually decline,many precious is limited by the types of promotion.Through artificial tissue culture technology,can effectively solve the problem of meaty plant breeding,have the effect of rich varieties,accelerate the breeding,good for meaty plant germplasm resources preservation,reproduction and so on all is of great significance.Therefore,this study with large window of meaty plant hankage,vientiane,niang leaf,root and crown as explant.Via callus induction or direct adventitious bud induction approach,set up efficient in vitro regeneration system of the species.For meat plant rapid propagation and large-scale genetic breeding laid a theoretical foundation and technical support,the main research results are as follows:1 The establishment of hankage in vitro regeneration systemWith hankage(Crassulaceae)of the plant leaf,root and crown as explant,studied the influence hankage leaf in vitro regeneration,the root of in vitro regeneration and crown propagation of multiple factors.The experimental results show that:(1)Hankage blade callus induction of appropriate media for MS + 2 mg/L 6-BA + 0.1 mg/L NAA;Hankage blade adventitious bud induction best hormone combination ratio as follows:1.5 mg/L 6-BA + 0.1 mg/L NAA had the highest adventitious bud induction frequency could reach 90.05%,the average number of adventitious bud induction was 8.13±1.05;(2)Root of adventitious bud induction,MS + 0.6 mg/L 6-BA + 0.1 mg/L NAA for hankage root callus induction of appropriate media;Adventitious bud induction of hormone combination optimal proportion for:2 mg/L 6-BA + 0.2 mg/L NAA,the highest adventitious bud induction frequency,can amount to 95.16%,the average number of adventitious bud induction was 8.16 ± 1.45;(3)The crown of adventitious bud induction,adventitious bud induction ratio of hormone combination is:0.4 mg/L 6-BA + 0.01 mg/L NAA,the average number of adventitious bud induction was 8.36 ± 1.54;(4)In all large window of adventitious bud rooting,0.6 mg/L IBA for large window vientiane regeneration seedling is most suited to take root density.2 The establishment of a large window phenomena in vitro regeneration system With large window Vientiane(laworthia maughanii)of the plant leafroot and crown as explant,studied the impact large window leaf in vitro regeneration,root in vitro regeneration and crown propagation of multiple factors,the establishment of a large window vientiane regeneration system research.The experimental results show that(1)All large window leaf in vitro regeneration,MS + 0.4 mg/L 6-BA + 0.1 mg/L NAA for large window Vientiane blade callus induction of appropriate media;Leaves adventitious bud induction hormone best mix proportion is:2 mg/L 6-BA+ 0.4 mg/LNAA.Under the condition of the hormones,the induction frequency of adventitious bud,highest can amount to 92.45%,the average number of adventitious bud induction was 8.361 1.34;(2)The big window vientiane root in vitro regeneration,MS+ 1 mg/L 6-BA + 0.05 mg/L NAA for big window vientiane root callus induction of appropriate media;2 mg/L 6-BA + 0,1 mg/L NAA for large window vientiane root best of adventitious bud differentiation media;(3)In the big window in vientiane crown propagation,best of all large window leaves adventitious bud induction hormone combination ratio is 1 mg/L 6-BA + 0.2 mg/L NAA,the average number of adventitious bud induction was 8.38± 1.54;(4)In vitro shoot begins to take root,0.3 mg/L IBA is hankage regeneration seedling is most suited to take root density.3 The establishment of the blessed mother in vitro regeneration systemIn Cotyledon orbiculata var.interi of the plant leaf,root and crown as explant,studied the influence f niang leaf in vitro regeneration and crown propagation of multiple factors.The experimental results show that,(1)Fu niang leaf in vitro regeneration,MS + 0.8 mg/L 6-BA +0.2 mg/L NAA for f niang blade callus induction of appropriate media;0.8 mg/L 6-BA + 0.8 mg/L NAA combination under the blessed mother leaves adventitious bud induction the best combination of plant hormones,the average number of adventitious bud induction up to as high as 8.33 ± 1.05;(2)Fu niang crown propagation,1 mg/L 6-BA + 0.05 mg/L NAA combination for differentiation f niang crown propagation under optimum plant hormone combination of multiple shoot clumps.The average number of adventitious bud induction up to as high as 8.06 +/-1.24,and the induction of adventitious bud status is good.(3)In blessing fu niang adventitious bud rooting,0.2 mg/L IBA blessed niang crown propagation regeneration seedling is most suited to take root density,obtained a better effect on rooting.