Analysis Of Optimal Cut-off Point For OGTT Diagnosis Of Abnormal Glucose Metabolism In Canine And Modeling Of Alloxan Diabetes In Canine

This paper based on the different dog group of oral glucose tolerance test(OGTT),to search a different group of blood glucose range at different time point after OGTT load,cut-off point of diagnosis of impaired glucose regulation(IGR)and diabetes mellitus(DM).And attempt to establish a canine diabetes model in order to further understand the pathogenesis of canine diabetes and the study of diabetes system modeling reference in the future.1 、 Analysis of Optimal Cut-Off Point for OGTT Diagnosis of Abnormal Glucose Metabolism in Canine150 test dogs were subjected to an OGTT test after fasting samples were collected.Time was taken from sugar was taken,and blood glucose was measured at 0.5h,1h,2h,and 3h after serving glucose.All the data obtained are filled in the EXCEL,and the blood glucose curve is produced.The SPSS 19.0 statistical software is used for calculation and analysis.All the measurement data are represented byˉx±S,and the range of 99% normal glucose reference value at different time points is calculated withˉx±2.58 S.The application of the Receiver Operating Characteristic(ROC)was used to analyze the tangent point of diagnosing canine diabetes at different moments.The results are 1.The canine normal blood glucose range : FPG is between3.53-6.73mmol/L and 2hPG <7.19mmol/L.2.The two-hour glucose value after oral glucose tolerance test is most significant in diagnosing canine glucose metabolism abnormality,and the best cut-point for diagnosing canine glucose metabolism abnormality at this time is 12.625mmol/L;the cut-points in the diagnosis of canine glucose metabolism abnormality at other times of OGTT were 13.845mmol/L for0.5hPG and 14.325mmol/L for 1hPG respectively.2、Analysis of Canine Alloxan Diabetes Mellitus ModelingThe experimental dogs were fasted for 24 hours before modeling.Weighed andestablished a vein channel through the forearm vein.Blood was taken from the channel to measure the normal fasting blood glucose value and other diabetic evaluation indexes.After that,the alloxan solution was injected rapidly at 60 mg/kg within 30 s and washed embedded indwelling needle with saline afterwards.Blood glucose value was measured at 2h,4h,10 h,24h,36 h,48h,and 72 h after injection,and drinking water was freely available.Two days later,the dogs were allowed to eat freely.The food was put away at 8.00 pm and blood was taken at 8.00 am the next day to measure the fasting blood glucose level.The experimental dogs were observed for their mental status,diet,drinking water,and urination.After administration,the fasting blood glucose and other diabetic evaluation indexes were measured once a week and weighed and recorded.After 48 hours,randomized blood glucose ≥11.11 mmol/L and maintained at this level for 2 weeks indicated that the diabetic dog was successfully modeled.Changes in liver and kidney pancreas biochemical parameters before and after administration were measured,and relevant tissue samples(liver,kidney,pancreas)were collected to make pathological sections.SPSS17.0 statistical software was used for calculation and analysis.The data were expressed as mean and standard deviation withˉx±S,and the significant difference was analyzed by one-way linear regression.The result is that A canine model of diabetes was successfully established by intravenous injection of alloxan 60 mg/kg.At the same time,it was also found that alloxan caused damage to the liver,kidney and pancreas of dogs at different doses.Therefore,it is recommended that if a model of canine diabetes is established using alloxan,a dose range of 30 mg/kg to 60 mg/kg can be referenced,and multiple doses of small doses can also be used to avoid toxic effects and reduce overall mortality.