The Investigation Of Trematode In Galba Pervia And Morphology Development Observation Of Echinostoma Revolutum In Duck Intestine

Food-borne trematodiases is a disease that can make serious damage for human and animals.These parasites contain Fasciolidae,Opisthorchiidae,Echinostomatidae and Heterophyidae and so on.It do harm for the health of human and animals after infection,and impact the factitive and commercial competence,thus to cause the economic losses.General,the prevalence and spread of these disease require 1 or 2 intermediate host.The first intermediate hosts are freshwater snails,and the second intermediate hosts are freshwater fishes.Therefore to survey the kinds of trematodes parasitism in the intermediate hosts is helpful for preventing the disease.In order to make sure the trematode species in the Galba pervia of Guangxi,during 2013 to 2014,we collected Galba pervia from 18 sites of Nanning,Liuzhou and Guilin.Crush the samples to find the trematode larvas.PCR amplificate the second internal transcribed spacer(ITS2)to identify.Feed ducklings with metacarcerias found in G pervia to acquire adults and base on morphology and molecular method to identify.The results showed that there are 6 spieces trematode found in the G pervia,4 of 6 were identified:the first is Echinostoma friedi,found in Tianbao reservoir;the second is Hypoderaeum conoideum,found in Hede,Liushan and Liutang;the third is E.revolutum,found in Guangrong;the last one is Australapatemon cf.burti.But the number of the 2 remaining spieces are too lack to identify.The results suggest that G.pervia from Nanning,Liuzhou and Guilin are intermediate host of various trematode,the H.conoideum is the most widespread spiece.Also the ITS2 is a reliable molecular marker to identify trematode.Additional,feed 10 ducklings with matecarceria of E.chinostoma to observe its development.Feces examination 8 days post infection.The results showed that the development of E.chinostoma was very fast in gut.The testis was observed 3 days post infection,the utero and cirrus sac were observed 4 days post infection,the parasite migrated to cecum 8 days post infection and the first appearance of eggs in utero and feces was on the 9th and 10th day after infection,respectively.