The Synthesis And Biological Application Of Organic Fluorescent Dye Porphyrins

Porphyrins have been well applied in biomedical applications due to their relatively high molar extinction coefficient,strong photostability and chemical stability.This article focuses on the use of porphyrin as a photosensitizer to achieve anti-tumor treatment through photothermal therapy and photodynamic therapy.Therefore,a porphyrin-HSA system with enhanced long-circulation ability in vivo and a porphyrin-ferrocene system for photodynamic and chemical kinetics combined treatment were constructed around functionalized TPP.A series of porphyrin photosensitizers?TPP-C_n?with different carbon chain lengths were designed and synthesized,then TPP-C_n and human serum albumin?HSA?self-assembled to form nanoparticles?TPP-C_n@HSA?.The particle size and potential of the prepared nanoparticles were tested and found to have good stability.The in vitro light heating curve experiment shows that with the increase of carbon chain length,the warming effect of TPP-C_n is better,and the photothermal conversion coefficient is higher(the photothermal conversion coefficient of TPP-C₁₆ is 45%).Moreover,after HSA was combined,the endocytosis of the cells was improved,and the photothermal treatment effect was studied at the cell level.It was found that these nanoparticles had good cytocompatibility,and the cells treated with TPP-C₁₆ had the lowest survival rate after illumination.The phototoxicity is the largest.A mouse tumor model was established and a tumor suppression experiment was performed.The results showed that TPP-C₁₆@HSA has the best anti-tumor effect in vivo,and can basically achieve complete tumor suppression.A near-infrared fluorescent complex?TNFC?consisting of a near-infrared fluorescent dye TPP-NH₂ and a Fenton reagent dicarboxyferrocene?FC?was designed and synthesized.Chemical kinetic treatment can generate oxygen?O₂?by reacting with hydrogen peroxide,solve the problem of tissue hypoxia,and improve the effect of photodynamic therapy.However,chemical kinetic treatment is affected by glutathione?GSH?in the body.The result of photodynamic therapy will reduce the GSH content in the cells,thereby avoiding the capture of hydroxyl radicals?.OH?and thus significantly improving the therapeutic effect of FC.In this paper,the ability of TNCF to produce ROS was studied by introducing salicylic acid and 1,3-diphenylisobenzofuran?DPBF?as indicators respectively.The introduction of DCFH-DA as an indicator at the cellular level demonstrated the production of ROS.Finally,the ability of TNCF to kill cancer cells was studied by MTT assay.The results showed that TNCF ultimately achieved synergistic treatment of photodynamic therapy?PDT?and chemodynamic therapy?CDT?.