Preparation And Identification Of Blood Pressure Lowering Polypeptide And Hypolipidemic Polypeptide In Camel Blood

Developed a camel blood product for multi-dimensional development,developed a new camel blood peptide with hypotensive and hypolipidemic functions,improved the utilization rate of discarded resources of the agricultural and sideline processing industry,and became a preventive and adjuvant treatment of hypertension for the camel blood peptide product.The high blood lipid functional health care product provides scientific basis,and also provides technical support for the promotion and application of the camel blood peptide product.In this experiment,peptides were prepared by enzymatic hydrolysis of proteases,and the complex protease,flavor protease,papain,pepsin,trypsin,acid protease,neutral protease,alkaline protease,pepsin and trypsin were common in the market.The effect of enzymatic hydrolysis was determined by using in vitro simulated gastrointestinal digestion to crudely lower blood pressure and lowering blood lipid polypeptides;purifying blood pressure lowering and lowering lipid peptides by ultrafiltration and Sephadex-25 dextran gel chromatography,and measuring and collecting The components with the best blood pressure lowering and lowering blood lipids were analyzed.The amino acid composition of blood pressure lowering and lowering blood lipid polypeptide was analyzed by amino acid automatic analyzer.The molecular weight and amino acid of blood pressure lowering and lowering blood lipid polypeptide were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,sequence.The main test results are as follows:(1)The selection of the optimal enzyme indicates that the camel blood is pretreated by ultrasonication,dialysis,etc.,and the substrate concentration is 5%and the enzyme addition amount is 5%under the action of pepsin and trypsin.At the optimum temperature and optimum pH,the macromolecular protein in the sample can be hydrolyzed into a small molecule polypeptide under the condition of 4 h hydrolysis,and the ACE inhibition rate and HMG-CoA reductase inhibition rate are higher than other.Enzymatic solution.(2)Purification of lowering blood pressure and lowering blood lipid polypeptides showed that the molecular weight of 3 kDa ultrafiltration tube was less than 3 kDa,and the ACE inhibition rate was 61.8%;the 3 kDa and 10 kDa super The polypeptide solution with a molecular weight of 3 kDa-10 kDa obtained by the filter tube has a high HMG-CoA reductase inhibition rate,and the HMG-CoA reductase inhibition rate is 11%.(3)In the identification of blood pressure-lowering and lowering blood lipid polypeptides in camel blood,the molecular weight of blood pressure lowering polypeptide is 600 Da-1756 Da,the amino acid sequences are NPNNR,VVDMPCTR,VDEVGWALGR;the molecular weight of hypolipidemic polypeptide is 4114.66 Da-4387.3164 Da,and its amino acid sequence for RVADEVGGEAIGR,EAVAAHHPGDFTPDAH,PDDDHGPGLNHLNNNK.