Study On Extraction Process Of Paeonol From Cortex Moutan And Fermentation Of Its Residue

New techniques and methods have been applied for the extraction of traditional Chinese medicines to improve their extraction rate of the active ingredients.Due to the complexity of the components of the materials,these techniques have certain drawbacks during the application.Thus,novel,scientific and modern methods to improve the extraction rate of the active ingredients have always been hot spots in Chinese medicine researches.Meanwhile,considering the low active ingredients content and the huge amount of residue after extraction,great environmental problems and wasting of resources will be caused if the post-processing works can’t be done properly.In this paper,pretreatment,extraction process and residue fermentation was studied to explore the efficient cleaning and utilization of cortex moutan.The obstruction during the paeonol extraction mainly derives from the cell wall and the cellulose layer.A novel plant-cell wall breaking technology,in-situ SO₃ micro thermal explosion technology was used to destruct the obstruction from the plant cell wall,following steam distillation extraction technology to extract the cortex moutan.Industrial analyzing methods,including H-NMR and HPLC was used to characterize the content and structure of the products.Results showed that the atmospheric SO₃micro-thermal explosion technology increased the extraction rate with an increase of 13%without damaging its quality and the purity of the product was 99%.At the same time,the microscopic imaging of the microstructure of the cortex moutan showed that the sieve tube and the epidermis burst due to the effect of SO₃.Analysis of the influential factors of the process on the extraction rate of paeonol showed that the increase was mainly caused by the SO₃ micro-thermal explosion.The extraction conditions were optimized and an optimal condition was obtained with a soaking time of 2.5 h,a particle size of 20 mesh and a distillation time of 2.5 h,with an extraction rate of 97%.This new process has obvious technical and economic advantages compared with other processes.Researches have been conducted to study the comprehensive utilization of the cortex moutan residue after the extraction of paeonol:1.the main components of the cortex moutan residue was analyzed and a strain that can directly use the cortex moutan residue to produce microbial oil was screened.The morphology and genes of the strain was identifiedand the results showed it was Talaromyces radicus.The response surface method was used for the optimization of the fermentation conditions.The yield of oil production by fermentation is up to 6%,among which unsaturated fatty acid linoleic acid accounts for about 90%,and saturated fatty acid palmitic acid accounts for about10%,which has great application value.2.The production of protein feed is carried out combining strains,and the optimal fermentation conditions are determined through optimization of strain combination,fermentation conditions and raw material processing.A high-energy protein feed with a crude protein content of up to 33%and crude fiber content of only 9.5%was obtained,which was proved to be feasible compared with feed standards.A double lifting of the reduction on amount and high value of the cortex moutan residue was achieved due to the specificity of the strain.