| The(meth)acrylate(amide)of multifunctional polymers which have many good characteristics,such as simple synthesis method,easily obtained materials,new and diverse function have aroused people’s widespread interest.Five kinds of acrylates monomers were designed and synthesized and have the function of antifouling,adhesion and derivative.The polymers which have the above functions were obtained by free-radical polymerization.The polymer coatings on stainless steel,silicon and gold surfaces were prepared by simple spin-coating or dip-coating methods.The effect of anti-protein adsorption on surfaces was also investigated.The contents are listed in following:Firstly,the overview of the development of multifunctional polymer;a brief introduction of the application of dopamine and fluorine functional groups in the field of polymer materials;a summary of several types of antifouling materials;an outline of the polymerization of polymers and the characterization of polymer coatings.Secondly,the design,synthesis and characterization of functional monomers and multifunctional polymers.We designed and synthesised five functional monemers,lipoic acid(L),amino group(A),azide(Z),dopamine(D)and carboxy(C),their molecular structures were confirmed by ’H NMR and FTIR.AIBN or V50 as the initiator,multifunctional polymers which has the function of antifouling,adhesion on surfaces and derivative were obtained by free-radical polymerization.1H NMR,FTIR,GPC,DLS,DSC and Tg etc methods were used to investigate the composition of the polymer,molecular weight distribution,particle size distribution,thermal gravimetric analysis etc.Their polymer coatings on stainless steel,gold and silicon surfaces were prepared by dipping or spin-coating methods,the properties of the morphology and wettability of the coatings on stainless steel and silicon were investigated by CA and SEM.Thirdly,our work reports a study of the properties of the polymer coatings which contain dopamine(D),oligomeric ethylene glycol(O)and perfluorinated alkyl(F)at varying ratios on stainless steel,silicon and gold substrate and the application of the coatings in anti-protein adsorption behavior.CA,XPS,AFM,GIR were used to test the morphology and surface wettability of the polymer films;bovine serum albumin(BSA)and fibrinogen(Fib)were used as model proteins adsorbed on surfaces to research the antifouling properties of the polymer coatings,the quantitative and qualitative intuitive analysis of protein adsorbed on gold or stainless steel surfaces were conducted by SPR and inverted fluorescence microscope.Results revealed that,the DOF164 surface showed a strong antifouling property towards the protein with the minimum absorption amount of BSA and Fib were 38.7 ng/cm2 and 43.5 ng/cm2 respectively;whereas the DOF1612 surface showed a high adsorption capacity to protein with the adsorbtion amount of BSA and Fib up to 157 ng/cm2 and 644 ng/cm2,12.3 folds higher as compared to the DOF164 surface.The conformational analysis of Fib protein and ELISA experiment revealed that the DOF164 surface can maintain best biological activity of the Fib protein.The relative activity of adsorbed Fib is measured to be 26.3 and 8.7 folds of that on DOF1612 and pure OEG surfaces,while the latter has been widely used as matrixes for non-fouling selective protein immobilization.The special feature of the DOF164 surface that has the least adsorption capacity yet the highest ability to maintain protein activity suggests the potential application as non-fouling biomaterial surface materials and the matrix for selective protein immobilization in biosensor development.This finding has guiding significance for the construction of new anti-fouling materials. |
PDF Full Text Request