| This study selected Lentinus edodes as a raw material,used enzyme-chemical methodology to optimize the Lentinus edodes dietary fiber(Lentinus edodes dietary fiber,LDF)extraction process,used the enzymatic modification to its optimized process,the extraction and modification of LDF,at the same time,assessed the extraction and modification of functional properties of dietary fibre in swelling capacity(SC),water hold capacity(WHC),oil hold capacity(OHC),cation exchange capacity(CEC),glucose adsorption capability(GAC),cholesterol absorption capacity(CAC).The results were as followed:1.Selected neutral protease dosage(0.2% ~ 0.8%),neutral protease digestion time(3 h~6h),NaOH addition(5%~14%),NaOH extraction time(1 h~2.5 h),NaOH extraction temperature(40?C~70?C),solid-liquid ratio(1:16~1: 28)this six factors to design orthogonal test.The optimum extraction conditions for the dietary fiber of Lentinus edodes were as followed: neutral protease dosage 0.4%;neutral protease digestion time 5 h;NaOH addition 11%;NaOH extraction time 2 h;NaOH extraction temperature 60?C;solid-liquid ratio 1:20,under these conditions The LDF yield was40.3%.2.Selected cellulase addition(0.5% ~ 2.5%),hydrolysis time(30 min ~ 150min),pH(4.5 ~ 6.5),hydrolysis temperature(40?C ~60?C),solid to liquid ratio(1:15~1:35)as a single factor.Selected the cellulase dosage,the enzymolysis time,and the ratio of solid and liquid to design a response surface test.The optimum technological conditions for the modification of dietary fiber of Lentinus edodes are:1.5% cellulase addition;enzymatic hydrolysis time 120 min;enzymolysis temperature50?C;pH 5.5;solid-liquid ratio 1:25.Under these conditions,the soluble dietary fiber(SDF)yield was 6.35%.3.Determination of the functional properties of extracted and modified LDF:(1)Swelling Capacity: The original SDF was 1.60 mL/g,the original insoluble dietary fiber(IDF)was 0 mL/g,and the modified SDF was 2.40 mL/g,the modified IDF 2.35mL/g,The modified SDF expandability is increased by 50%,the modified IDF expansivity by nearly 2 times.(2)Water Holding Capacity:The original SDF was 8.53g/g,the original IDF was 13.03 g/g,the modified SDF was 10.39 g/g,the modified IDF was 13.15 g/g,and the modified SDF water holding capacity was increased by21.8%.The water holding capacity of sexual IDF was increased by 0.9%.(3)Oil Hold Capacity: the original SDF was 5.67 g/g,the original IDF was 8.79 g/g,and the modified SDF was 9.10 g/g,the modified IDF was 10.07 g/g,the modified SDF oil hold capacityincreased was increased by 60.5%,the modified IDF oil hold capacityincreased was increased by 14.6%,physical properties of modified dietary fiber were all higher than those before modification.(4)Cation Exchange Capacity:in the entire process of adding 0-6.0 mL of NaOH,the pH of the original SDF solution changes by 9.82,and the pH of the modified SDF solution Changed 9.75,original pH of IDF the solution was changed by 10.02,the pH of the modified IDF solution was changed by 9.80,the modified SDF cation exchange capacity was increased by 0.7%,and the modified IDF cation exchange capacity was increased by 2.24%.(5)Glucose Adsorption Capability:with the increase of glucose concentration,the glucose adsorption capacity of the extracted and modified dietary fiber is increasing.(6)Cholesterol Absorption Capacity:the original SDF was 0.95 mg/g,the modified SDF was 2.83 mg/g,the original IDF was 0.17 mg/g,the modified IDF was 0.54 mg/g,and the modified SDF cholesterol adsorption capacity was increased by nearly 2 times.Modified IDF cholesterol adsorption capacity increased more than 2 times.Through the extraction and modification of the functional properties of dietary fiber,it was found that LDF has better functional properties. |
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